CAJ | 학술논문

以马铃薯(Solanum tuberosum)为实验材料,利用电子克隆和RACE技术,从马铃薯中克隆出NOA(nitric oxide associated factor)基因,命名为StNOA1,测序结果表明,其cDNA序列长度为1 929 bp,此片段包含一个长为1 632 bp的完整编码框.氨基酸序列比对分析表明,StNOA1与烟草(Nicotiana benthamiana),葡萄(Vitis vinifera),蓖麻(Ricinus communis),水稻(Oryza sativa),玉米(Zea mays)以及拟南芥(Arabidopsis thaliana)均有很高的同源性 (89.44%～63.56%).同AtNOA1一样,StNOA1也具有保守的GTP结合区.从结构分析结果推测,StNOA1和AtNOA1在功能上有一定的相关性,其也可能通过调节内源NO的释放参与到植物生长、发育、抗逆等过程中.
이마령서(Solanum tuberosum)위실험재료,이용전자극륭화RACE기술,종마령서중극륭출NOA(nitric oxide associated factor)기인,명명위StNOA1,측서결과표명,기cDNA서렬장도위1 929 bp,차편단포함일개장위1 632 bp적완정편마광.안기산서렬비대분석표명,StNOA1여연초(Nicotiana benthamiana),포도(Vitis vinifera),비마(Ricinus communis),수도(Oryza sativa),옥미(Zea mays)이급의남개(Arabidopsis thaliana)균유흔고적동원성 (89.44%～63.56%).동AtNOA1일양,StNOA1야구유보수적GTP결합구.종결구분석결과추측,StNOA1화AtNOA1재공능상유일정적상관성,기야가능통과조절내원NO적석방삼여도식물생장、발육、항역등과정중.
The NOA (nitric oxide associated factor) gene encodes a NOA which participates in nitric oxide synthase (NOS) dependent NO synthesis.In this study,the cDNA of StNOA1 was cloned by in silico cloning and combination with RACE from potato (Solanum tuberosum).The full length of StNOA1 cDNA had 1 929 bp with an open reading frame of 1 632 bp.Phylogenetic analysis revealed that the StNOA1 amino acid sequence shared high identity (89.44%～63.56%) with the NOA from Nicotiana benthamiana,Vitis vinifera,Ricinus communis,Oryza sativa,Zea mays and Arabidopsis thaliana.The StNOA1 bears a centrally positioned GTPase-binding domain as well as AtNOA1.Its sequence conservation and structure similarity with AtNOA1 implied the functional correlation between StNOA1 and AtNOA1 in plant growth,development,and responses to stresses and pathologies by regulating endogenous NO production.