上海交通大学学报（医学版）
上海交通大學學報（醫學版）
상해교통대학학보（의학판）
JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY(MEDICAL SCIENCE)
2009年
11期
1331-1335
,共5页
全氟化碳%双层法%心肌保护%能量代谢%大鼠
全氟化碳%雙層法%心肌保護%能量代謝%大鼠
전불화탄%쌍층법%심기보호%능량대사%대서
perfluorochemical%two-layer method%heart preservation%energy metabolism%rat
目的 研究全氟化碳化合物(PFC)在大鼠离体心脏长时程低温保存中的作用.方法 30只Wistar大鼠随机分为对照组(Celsior保存液)、Celsior 液通氧组(Celsior+O_2)、双层法通氧组(PFC/Celsior+O_2),每组10只.制备大鼠离体心脏Langendorff灌注模型.离体心脏在4 ℃条件下保存8 h,于复灌期分别检测血流动力学指标、冠脉流出量及磷酸肌酸激酶(CK) 、乳酸脱氢酶(LDH) 和天门冬氨酸转氨酶(AST)漏出量,观察心肌超微结构的改变.结果 心脏复灌后,与对照组和Celsior+O_2组比较,Celsior/PFC+O_2组左心室舒张压和左心室压力变化速率明显上升(P<0.01),LDH、CK和AST漏出量明显减少(P<0.01);而Celsior+O_2组与对照组上述指标比较差异均无统计学意义(P>0.05).超微结构观察发现,与对照组和Celsior+O_2组比较,低温保存后PFC/Celsior+O_2组心肌纤维排列较整齐,肌浆网扩张、线粒体肿胀及嵴结构破坏程度均较轻.结论 氧合的PFC与常规心脏保存液联合应用能够通过向缺血心肌提供更多的氧来改善能量代谢,提高离体大鼠心脏长时程低温保存的效果.
目的 研究全氟化碳化閤物(PFC)在大鼠離體心髒長時程低溫保存中的作用.方法 30隻Wistar大鼠隨機分為對照組(Celsior保存液)、Celsior 液通氧組(Celsior+O_2)、雙層法通氧組(PFC/Celsior+O_2),每組10隻.製備大鼠離體心髒Langendorff灌註模型.離體心髒在4 ℃條件下保存8 h,于複灌期分彆檢測血流動力學指標、冠脈流齣量及燐痠肌痠激酶(CK) 、乳痠脫氫酶(LDH) 和天門鼕氨痠轉氨酶(AST)漏齣量,觀察心肌超微結構的改變.結果 心髒複灌後,與對照組和Celsior+O_2組比較,Celsior/PFC+O_2組左心室舒張壓和左心室壓力變化速率明顯上升(P<0.01),LDH、CK和AST漏齣量明顯減少(P<0.01);而Celsior+O_2組與對照組上述指標比較差異均無統計學意義(P>0.05).超微結構觀察髮現,與對照組和Celsior+O_2組比較,低溫保存後PFC/Celsior+O_2組心肌纖維排列較整齊,肌漿網擴張、線粒體腫脹及嵴結構破壞程度均較輕.結論 氧閤的PFC與常規心髒保存液聯閤應用能夠通過嚮缺血心肌提供更多的氧來改善能量代謝,提高離體大鼠心髒長時程低溫保存的效果.
목적 연구전불화탄화합물(PFC)재대서리체심장장시정저온보존중적작용.방법 30지Wistar대서수궤분위대조조(Celsior보존액)、Celsior 액통양조(Celsior+O_2)、쌍층법통양조(PFC/Celsior+O_2),매조10지.제비대서리체심장Langendorff관주모형.리체심장재4 ℃조건하보존8 h,우복관기분별검측혈류동역학지표、관맥류출량급린산기산격매(CK) 、유산탈경매(LDH) 화천문동안산전안매(AST)루출량,관찰심기초미결구적개변.결과 심장복관후,여대조조화Celsior+O_2조비교,Celsior/PFC+O_2조좌심실서장압화좌심실압력변화속솔명현상승(P<0.01),LDH、CK화AST루출량명현감소(P<0.01);이Celsior+O_2조여대조조상술지표비교차이균무통계학의의(P>0.05).초미결구관찰발현,여대조조화Celsior+O_2조비교,저온보존후PFC/Celsior+O_2조심기섬유배렬교정제,기장망확장、선립체종창급척결구파배정도균교경.결론 양합적PFC여상규심장보존액연합응용능구통과향결혈심기제공경다적양래개선능량대사,제고리체대서심장장시정저온보존적효과.
Objective To investigate the effects of perfluorochemical ( PFC) on in vitro long-term hypothermic heart preservation in rats. Methods Thirty Wistar rats were randomly divided into control group ( Celsior solution), Celsior + O_2 group (Celsior solution plus oxygen) and PFC/Celsior + O_2 group (two-layer method plus oxygen) , with 10 rats in each group. Langendorff model of isolated rat heart was prepared. The isolated heart was preserved at 4 ℃ for 8 h, and hemodynamic parameters, coronary effluent flow, and leakage of creatine kinase ( CK), lactate dehydrogenase ( LDH) and aspartate transaminase (AST) were detected after reperfusion. Besides, the myocardial ultrastructure was also observed. Results Compared with control group and Celsior + O_2 group, the left ventricular developed pressure and ± dp/dt in PFC/ Celsior + O_2 group significantly increased ( P < 0.01), while LDH, CK and AST leakage significantly decreased ( P < 0.01). However, there was no significant difference in the above paramters between Celsior + O_2 group and control group (P>0.05). Compared with control group and Celsior + O_2 group, the impairment of myocardial ultrastructure in PFC/ Celsior + O_2 group after hypothermic preservation was alleviated. Conclusion PFC as a supplementation to oxygen in heart preservation solution could enhance myocardial protection during in vitro long-term hypothermic heart preservation in rats via the improvement of energy metabolism.
