CAJ | 학술논문

目的:研究抗氧化剂N-乙酰半胱氨酸(NAC)对小鼠肝脏缺血再灌注损伤模型中肝肺组织的保护作用及机制.方法:制备BALB/c小鼠肝部分缺血再灌注损伤模型, 将小鼠随机分为3组: 假手术组(SH组), 缺血再灌注组(I/R组)和NAC组(I/R-NAC组).分别于再灌注后1 h、 3 h取静脉血测定血清TNF-α浓度和ALT水平;取肺组织标本测湿干质量比及病理.同时取肝、肺组织行RT-PCR以观测Toll样受体2/4(TLR2/4)表达.结果:肺组织病理结果显示I/R组肺组织毛细血管充血, 肺泡结构破坏, 肺泡间质中性粒细胞浸润, 肺泡腔内不同程度渗出及少量红细胞.肺湿干质量比显示肺水肿.缺血再灌注后静脉血清TNF-α浓度和ALT水平较假手术组显著升高.受损肝叶和肺组织内均出现TLR2/4 mRNA高表达, NAC干预后, 肺水肿明显减轻;TLR2/4 mRNA表达受到抑制;血清TNF-α浓度和ALT水平较I/R组明显下降.结论:N-乙酰半胱氨酸抑制再灌注后TLR2/4的活化, 降低TNF-α的分泌, 从而减轻缺血再灌注肝、肺组织的损伤.
목적:연구항양화제N-을선반광안산(NAC)대소서간장결혈재관주손상모형중간폐조직적보호작용급궤제.방법:제비BALB/c소서간부분결혈재관주손상모형, 장소서수궤분위3조: 가수술조(SH조), 결혈재관주조(I/R조)화NAC조(I/R-NAC조).분별우재관주후1 h、 3 h취정맥혈측정혈청TNF-α농도화ALT수평;취폐조직표본측습간질량비급병리.동시취간、폐조직행RT-PCR이관측Toll양수체2/4(TLR2/4)표체.결과:폐조직병리결과현시I/R조폐조직모세혈관충혈, 폐포결구파배, 폐포간질중성립세포침윤, 폐포강내불동정도삼출급소량홍세포.폐습간질량비현시폐수종.결혈재관주후정맥혈청TNF-α농도화ALT수평교가수술조현저승고.수손간협화폐조직내균출현TLR2/4 mRNA고표체, NAC간예후, 폐수종명현감경;TLR2/4 mRNA표체수도억제;혈청TNF-α농도화ALT수평교I/R조명현하강.결론:N-을선반광안산억제재관주후TLR2/4적활화, 강저TNF-α적분비, 종이감경결혈재관주간、폐조직적손상.
AIM: To investigate protective effect of N-acetylcysteine (NAC) on liver and lung in mice after hepatic ischemia/reperfusion injury. METHODS: BALB/c mice were used in a model of partial hepatic ischemia/reperfusion (I/R) injury.They are divided randomly to sham-operated control group(SH), hepatic I/R group or NAC pretreated in hepatic I/R group(I/R-NAC).The level of TNF-α in protal vein and plasma ALT were measured at 1hour and 3 hour, respectively after reperfusion.Lung tissue wet-to-dry(W/D) weight ratio compared. RESULTS: Lung tissue W/D ratio showed significant difference between two groups; The expressions of TLR2/4 mRNA in liver and lung increased obviously after hepatic I/R injury. Histological evaluation showed several changes in lung tissue in I/R group.The level of TNF-α and ALT in protal vein increased continually in I/R group at 1hour and 3 hour of reputation compared with SH group.The level of TNF-α and ALT declined significantly in the group pretreated by NAC. CONCLUSION: N-acetylcysteine can inhibit the activation of TLR2/4 and reduce TNF-α secretion resulted from I/R injury it might abate liver and lung injury following partial hepatic ischemia-reperfusion in mice.