CAJ | 학술논문

从表现腺胃炎的尼克珊瑚粉商品代蛋鸡中分离到J亚群-白血病病毒(ALV-J).将病料或鸡白细胞接种于CEF,培养12 d,分别采用单克隆抗体间接免疫荧光试验检测,结果10只鸡中有9只鸡分离到ALV-J,其中有4只鸡还存在与禽网状内皮增生病病毒(REV)的共感染.通过PCR扩增gp85基因,与已发表的20株ALV-J进行同源性比较.结果表明,与来自白羽肉鸡的HPRS103的同源性为97.8%,而与来自蛋用型鸡的SD07LK1株的同源性为93.0%.本研究发现,在某些仅仅发生腺胃炎的鸡也可能普遍存在ALV-J感染,再次显示了腺胃炎病料中病毒感染的多样性.ALV-J可能成为致腺胃炎的病原之一,但其致病作用有待进一步研究.
종표현선위염적니극산호분상품대단계중분리도J아군-백혈병병독(ALV-J).장병료혹계백세포접충우CEF,배양12 d,분별채용단극륭항체간접면역형광시험검측,결과10지계중유9지계분리도ALV-J,기중유4지계환존재여금망상내피증생병병독(REV)적공감염.통과PCR확증gp85기인,여이발표적20주ALV-J진행동원성비교.결과표명,여래자백우육계적HPRS103적동원성위97.8%,이여래자단용형계적SD07LK1주적동원성위93.0%.본연구발현,재모사부부발생선위염적계야가능보편존재ALV-J감염,재차현시료선위염병료중병독감염적다양성.ALV-J가능성위치선위염적병원지일,단기치병작용유대진일보연구.
Subgroup J avian leukosis virus (ALV-J) was isolated from both provetriculus suspensions and blood samples of 9 in 10 laying hens only with proventriculitis, and co-infection of ALV-J with reticuloendotheliosis virus was also confirmed in 4 chickens. The gp85 genes of ALV-J isolates were amplified by PCR, sequenced and compared to 20 reference ALV-J strains.Sequence comparisons indicated that the provetriculus isolate had 97.8% identity with the HPPS103 strain isolated from white meat-type chickens and 93.0% identity with SD07LK1 strain from egg-type chickens. The result demonstrated that ALV-J infection was common in some chickens with only proventriculus, further indicating the diversity of virus infections associated with proventriculitis. ALV-J may be take part in pathogenesis of provetriculitis in some chickens,but its pathogenic role needs to be further studied.