时珍国医国药
時珍國醫國藥
시진국의국약
LISHIZHEN MEDICINE AND MATERIA MEDICA RESEARCH
2010年
3期
610-611
,共2页
王瑞%陈海云%杨琪伟%黄山君%王峥涛
王瑞%陳海雲%楊琪偉%黃山君%王崢濤
왕서%진해운%양기위%황산군%왕쟁도
独活%二氢欧山芹醇当归酸酯%高效液相色谱
獨活%二氫歐山芹醇噹歸痠酯%高效液相色譜
독활%이경구산근순당귀산지%고효액상색보
Radix Angelicae Pubescentis%Columbianadin%HPLC
目的 建立独活药材与饮片中二氢欧山芹醇当归酸酯的HPLC分析方法.方法 采用Shiseido C_(18) MG(4.6 mm × 250 mm,5μm)色谱柱,以乙腈-水(49∶51)为流动相,流速1 ml·min~(-1),检测波长330 nm,柱温30℃.结果 二氢欧山芹醇当归酸酯在0.003 6～1.536 mg·ml~(-1)呈良好的线性关系,r=0.999 9,平均回收率为99.1 %(RSD=2.8 %).22批不同产地独活药材与饮片中二氢欧山芹醇当归酸酯的质量分数在0.058 %～0.778 %,平均值0.178 %.结论 该方法简单、重复性好,可用于独活的质量控制.
目的 建立獨活藥材與飲片中二氫歐山芹醇噹歸痠酯的HPLC分析方法.方法 採用Shiseido C_(18) MG(4.6 mm × 250 mm,5μm)色譜柱,以乙腈-水(49∶51)為流動相,流速1 ml·min~(-1),檢測波長330 nm,柱溫30℃.結果 二氫歐山芹醇噹歸痠酯在0.003 6～1.536 mg·ml~(-1)呈良好的線性關繫,r=0.999 9,平均迴收率為99.1 %(RSD=2.8 %).22批不同產地獨活藥材與飲片中二氫歐山芹醇噹歸痠酯的質量分數在0.058 %～0.778 %,平均值0.178 %.結論 該方法簡單、重複性好,可用于獨活的質量控製.
목적 건립독활약재여음편중이경구산근순당귀산지적HPLC분석방법.방법 채용Shiseido C_(18) MG(4.6 mm × 250 mm,5μm)색보주,이을정-수(49∶51)위류동상,류속1 ml·min~(-1),검측파장330 nm,주온30℃.결과 이경구산근순당귀산지재0.003 6～1.536 mg·ml~(-1)정량호적선성관계,r=0.999 9,평균회수솔위99.1 %(RSD=2.8 %).22비불동산지독활약재여음편중이경구산근순당귀산지적질량분수재0.058 %～0.778 %,평균치0.178 %.결론 해방법간단、중복성호,가용우독활적질량공제.
Objective To develop a quantitative method for determination of columbianadin in Radix Angelicae Pubescentis. Methods The samples were separated at 30℃ on a Shiseido C_(18) MG (5 μm,4.6 mm×250 mm) column eluted with acetonitrile-water (49∶51) as the mobile phase. Flow rate was set at 1.0 ml·min~(-1) and the detection wavelength was set at 330 nm. Results The calibration curve was linear within the range from 0.0036 to 1.536 mg·ml~(-1) (r =0. 999 9) and the average recovery was 99.1 % (RSD=2.8 %). 22 batches of the drugs purchased from different areas were determined and the contents of columbianadin in Radix angelicae pubescentis were fluctuated from 0. 058 % to 0.778 %. Conclusion The method is simple, repeatable and can be used for the quality control of Radix Angelicae Pubescentis.
