一种快速微量提取柑橘早期胚基因组DNA的方法
일충쾌속미량제취감귤조기배기인조DNA적방법
A Rapid Method for the Isolation of Small Amount DNA from Citrus Early Embryo
  • 万方数据
    农业科学与技术(英文版) 농업과학여기술(영문판)
    AGRICULTURAL SCIENCE & TECHNOLOGY
    2010年 11期 95-97 ,共3页

    范达%雷天刚%王军政%宋二玲%彭爱红%谭洪泉%王金萍%李政利%李凤龙%陈勇%陈善春

    범체%뢰천강%왕군정%송이령%팽애홍%담홍천%왕금평%리정리%리봉룡%진용%진선춘

    柑橘基因组%CTAB法%微量提取%PCR 柑橘基因組%CTAB法%微量提取%PCR
    감귤기인조%CTAB법%미량제취%PCR
    Citrus genome%CTAB method%Isolation of small amount DNA%PCR
    [目的]建立一种快速微量提取柑橘基因组DNA的方法.[方法]采用优化的CTAB微量提取法,以20.0,10.0,5.0,2.5 mg的早期杂种胚为材料进行基因组DNA的提取,并对其DNA进行质量检测和SSR验证.[结果]该方法简单易行,所需材料少,提取的DNA纯度较高,OD260 nm/OD280 nm在1.800～2.000,可满足SSR等以PCR扩增为基础的试验需要.[结论]建立的方法可以用于快速微量提取柑橘基因组DNA.
    [목적]건립일충쾌속미량제취감귤기인조DNA적방법.[방법]채용우화적CTAB미량제취법,이20.0,10.0,5.0,2.5 mg적조기잡충배위재료진행기인조DNA적제취,병대기DNA진행질량검측화SSR험증.[결과]해방법간단역행,소수재료소,제취적DNA순도교고,OD260 nm/OD280 nm재1.800～2.000,가만족SSR등이PCR확증위기출적시험수요.[결론]건립적방법가이용우쾌속미량제취감귤기인조DNA.
    [Objective] The present study aimed to establish a rapid method for the isolation of small amount DNA from citrus.[Method] By using the improved CTBA method, the genomic DNA was extracted respectively from 20, 10, 5 and 2.5 mg hybrid embryos of citrus, and then the DNA quality was detected and followed by SSR verification.[Result] The method was very simple and rapid, which needed less materials.In addition, the isolated DNA showed good purity with the OD260/OD280 of 1.8-2.1, and could meet the requirement for PCR-based technology, such as SSR, etc..[Conclusion] The method could be used for rapid extraction of small amount of genomic DNA from citrus.
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