CAJ | 학술논문

目的研究非小细胞肺癌癌组织p16基因甲基化及其临床诊断意义.方法选择47例非小细胞肺癌患者,用MSP技术检测肺癌组织和癌旁正常组织p16基因甲基化.结果肺癌组织p16基因甲基化率44.7%,高于癌旁组织的17.0%(P＜0.01);肺癌组织甲基化检出率与临床分期、临床病理分型和临床分类之间差异无统计学意义(均P＞0.05).结论提示肺癌有关组织或样本的p16基因甲基化,可能成为各种临床类型的非小细胞肺癌早期诊断的一个有效指标.但特异性、灵敏性和可行性等有待进一步研究.
목적 연구비소세포폐암암조직p16기인갑기화급기림상진단의의.방법 선택47례비소세포폐암환자,용MSP기술검측폐암조직화암방정상조직p16기인갑기화.결과 폐암조직p16기인갑기화솔44.7%,고우암방조직적17.0%(P＜0.01);폐암조직갑기화검출솔여림상분기、림상병리분형화림상분류지간차이무통계학의의(균P＞0.05).결론 제시폐암유관조직혹양본적p16기인갑기화,가능성위각충림상류형적비소세포폐암조기진단적일개유효지표.단특이성、령민성화가행성등유대진일보연구.
Objective To detect methylation of p16 gene in lung cancer tissues of non-small cell lung cancer patients,and to approach its clinical diagnostic value.Methods The methylation of p16 gene in DNA from 47 non-small cell lung cancer tissues and corresponding nomalignant tissues were tested with methylation-specific PCR(MSP).Results The total frequency of p16 methylation was significantly higher in lung cancer tissues than that in the corresponding malignant tissue(44.7%vs 17%)(P＜0.01).But there was no significant difference in detectiveness,clinical staging,clinical pathology type and clinical classification(P＞0.05).Conclusion The detection of methylation of p16 gene may be helpful to clinical diagnosis for non-small cell lung cancer,but its specify,sensitivity and feasibility need to be further studied.