CAJ | 학술논문

目的探讨癌基因MDM2 mRNA在急性白血病(AL)中的过度表达及其临床意义.方法采用RT-PCR法检测了100例初诊及复发或难治AL患者和20名健康对照者MDM2基因的表达.采用χ2检验、t检验及单因素方差分析对结果进行统计学分析,以比较各组MDM2基因阳性率及表达强度差异.结果 100例患者中58例(58%)AL患者有较高水平的MDM2表达,明显高于对照组(P＜0.005);不良预后组28例中19例(67.9%)MDM2高表达,明显高于一般预后组[56例中19例MDM2高表达(33.9%)](P＜0.05).结论 MDM2基因过度表达在AL的发病机制及不良预后中起了重要的作用.
목적 탐토암기인MDM2 mRNA재급성백혈병(AL)중적과도표체급기림상의의.방법 채용RT-PCR법검측료100례초진급복발혹난치AL환자화20명건강대조자MDM2기인적표체.채용χ2검험、t검험급단인소방차분석대결과진행통계학분석,이비교각조MDM2기인양성솔급표체강도차이.결과 100례환자중58례(58%)AL환자유교고수평적MDM2표체,명현고우대조조(P＜0.005);불량예후조28례중19례(67.9%)MDM2고표체,명현고우일반예후조[56례중19례MDM2고표체(33.9%)](P＜0.05).결론 MDM2기인과도표체재AL적발병궤제급불량예후중기료중요적작용.
Objective To study the over-expression and clinical implications of the oncogene MDM2 in acute leukemia (AL). Methods The expression of MDM2 gene in 100 patients with newly diagnosed and relapse or refractory AL and 20 healthy as control was measured by relative quantitative reverse transcriptase polymerase chain reaction (RT-PCR),then the results was measured by χ2-test,t-test and one-way ANOVA to compare expession positive rate and intensity of MDM2. Results Among 100 patients,fifty-eight had the high expression of MDM2 gene (58 %). The expression level of MDM2 gene in patients was higher than that of health controls(P ＜0.05). The expression positive rate of MDM2 is higher in poor outcome group (67.9 %,19/28)than that in general outcome group (33.9%,19/56) (P＜0.05). Conclusion Our results suggest that the expression of MDM2 gene plays an important role in the pathogenesis and poor outcome of AL.