中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2010年
7期
878-880
,共3页
冯文明%朱鸣%鲍鹰%温晓红%费卯云%叶美娣%张晓岚%陶玉龙%蒋晓颖%王耀
馮文明%硃鳴%鮑鷹%溫曉紅%費卯雲%葉美娣%張曉嵐%陶玉龍%蔣曉穎%王耀
풍문명%주명%포응%온효홍%비묘운%협미제%장효람%도옥룡%장효영%왕요
受体,阿片样,δ%脓毒症%免疫,细胞
受體,阿片樣,δ%膿毒癥%免疫,細胞
수체,아편양,δ%농독증%면역,세포
Receptors,opioid,delta%Sepsis%Immunity,celluar
目的 探讨δ阿片受体激动剂对脓毒症大鼠细胞免疫功能的影响.方法 健康成年雄性SD大鼠150只,体重154～198g,随机分为3组(n=50):假手术组(S组)仅穿线,不进行盲肠结扎穿孔;脓毒症组(SEP组)采用盲肠结扎穿孔的方法制备脓毒症模型;δ阿片受体激动剂组(DADLE组)于盲肠结扎穿孔后立即腹腔注射δ阿片受体激动剂DALDE 10 ml/kg,浓度为0.5 mg/ml.于盲肠结扎穿孔后4、8、12 h(T1～3)时各组随机取10只大鼠,抽取下腔静脉血样,采用ELISA法检测血清TNF-α和IL-10的浓度,采用流式细胞仪检测T淋巴细胞亚群变化.记录盲肠结扎穿孔后7 d内大鼠的生存情况.结果 与S组比较,SEP组和DADLE组各时点血清TNF-α和IL-10的浓度、TNF-α/lL-10比值升高,T3时CD4+T细胞水平和CD4+/CD8+降低,CD8+T细胞水平升高(P＜0.05或0.01);与SEP组比较,DADLE组T2.3时血清TNF-α和IL-10的浓度、TNF-α/IL-10比值降低,T3时CD4+T细胞水平和CD4+/CD8+升高,CD8+T细胞水平降低,盲肠结扎穿孔后7 d内大鼠生存率升高(P＜0.05).结论 δ阿片受体激动剂可改善细胞免疫功能,从而减轻炎性反应,有利于脓毒症大鼠的预后.
目的 探討δ阿片受體激動劑對膿毒癥大鼠細胞免疫功能的影響.方法 健康成年雄性SD大鼠150隻,體重154～198g,隨機分為3組(n=50):假手術組(S組)僅穿線,不進行盲腸結扎穿孔;膿毒癥組(SEP組)採用盲腸結扎穿孔的方法製備膿毒癥模型;δ阿片受體激動劑組(DADLE組)于盲腸結扎穿孔後立即腹腔註射δ阿片受體激動劑DALDE 10 ml/kg,濃度為0.5 mg/ml.于盲腸結扎穿孔後4、8、12 h(T1～3)時各組隨機取10隻大鼠,抽取下腔靜脈血樣,採用ELISA法檢測血清TNF-α和IL-10的濃度,採用流式細胞儀檢測T淋巴細胞亞群變化.記錄盲腸結扎穿孔後7 d內大鼠的生存情況.結果 與S組比較,SEP組和DADLE組各時點血清TNF-α和IL-10的濃度、TNF-α/lL-10比值升高,T3時CD4+T細胞水平和CD4+/CD8+降低,CD8+T細胞水平升高(P＜0.05或0.01);與SEP組比較,DADLE組T2.3時血清TNF-α和IL-10的濃度、TNF-α/IL-10比值降低,T3時CD4+T細胞水平和CD4+/CD8+升高,CD8+T細胞水平降低,盲腸結扎穿孔後7 d內大鼠生存率升高(P＜0.05).結論 δ阿片受體激動劑可改善細胞免疫功能,從而減輕炎性反應,有利于膿毒癥大鼠的預後.
목적 탐토δ아편수체격동제대농독증대서세포면역공능적영향.방법 건강성년웅성SD대서150지,체중154～198g,수궤분위3조(n=50):가수술조(S조)부천선,불진행맹장결찰천공;농독증조(SEP조)채용맹장결찰천공적방법제비농독증모형;δ아편수체격동제조(DADLE조)우맹장결찰천공후립즉복강주사δ아편수체격동제DALDE 10 ml/kg,농도위0.5 mg/ml.우맹장결찰천공후4、8、12 h(T1～3)시각조수궤취10지대서,추취하강정맥혈양,채용ELISA법검측혈청TNF-α화IL-10적농도,채용류식세포의검측T림파세포아군변화.기록맹장결찰천공후7 d내대서적생존정황.결과 여S조비교,SEP조화DADLE조각시점혈청TNF-α화IL-10적농도、TNF-α/lL-10비치승고,T3시CD4+T세포수평화CD4+/CD8+강저,CD8+T세포수평승고(P＜0.05혹0.01);여SEP조비교,DADLE조T2.3시혈청TNF-α화IL-10적농도、TNF-α/IL-10비치강저,T3시CD4+T세포수평화CD4+/CD8+승고,CD8+T세포수평강저,맹장결찰천공후7 d내대서생존솔승고(P＜0.05).결론 δ아편수체격동제가개선세포면역공능,종이감경염성반응,유리우농독증대서적예후.
Objective To investigate the effect of δ-opioid receptor agonist D-Ala2-D-Leu5-enkephalin (DADLE) on the celluar immune function in septic rats. Methods One hundred and fifty healthy male SD rats weighing 154-198 g were randomly divided into 3 groups (n = 50 each):group Ⅰ sham operation (group S);group Ⅱ sepsis (group SEP) and group Ⅲ DADLE. Sepsis was induced by cecum ligation and punture (CLP) in group Ⅱ and Ⅲ. In group Ⅲ 0.5 rmg/kg DADLE 10 ml/kg was injected intraperitoneally (IP) immediately after CLP operation. Seven day survival rate was calculated. Blood samples were collected from 10 animals at 4, 8 and 12 h after operation (T1-3) respectively in each group for determination of serum TNF-α and IL-10 concentrations by ELISA and changes in T-cell subsets by flow cytometry. Results CLP significantly increased serum TNF-α and IL-10 concentrations and TNF-α/IL-10 ratio at T1-3 and decreased CD4+ and CD4+/CD8+ ratio and increased CD8+ at T3 in group Ⅱ as compared with group S (group Ⅰ). DADLE treatment significantly attenuated the CLP-induced above changes. Seven-day survival rate was significantly higher in DADLE group than in CLP group.Conclusion δ-opioid receptor agonist DADLE can improve the celluar immune function of rats with sepsis and increase the survival rate.
