中华妇产科杂志
中華婦產科雜誌
중화부산과잡지
CHINESE JOUNAL OF OBSTETRICS AND GYNECOLOGY
2009年
10期
754-759
,共6页
谷晓鸿%卢媛%马端%刘惜时%郭孙伟
穀曉鴻%盧媛%馬耑%劉惜時%郭孫偉
곡효홍%로원%마단%류석시%곽손위
卵巢肿瘤%DNA甲基化%CpG岛%肿瘤标记%生物学
卵巢腫瘤%DNA甲基化%CpG島%腫瘤標記%生物學
란소종류%DNA갑기화%CpG도%종류표기%생물학
Ovarian neoplasms%DNA methylation%CpG islands%Tumor markers%biological
目的 建立卵巢上皮性癌(卵巢癌)的DNA异常甲基化模式,探讨其在寻找新的卵巢癌特异性标志物中的应用价值.方法 用激光显微切割技术从20例卵巢癌组织冰冻切片中获取的肿瘤细胞作为实验对象,用原代培养的5例正常卵巢上皮细胞作为对照,用基于芯片技术的差异甲基化杂交(DMH)方法检测卵巢癌的DNA异常甲基化模式.选择7个DMH结果显示在卵巢癌中低甲基化的基因启动子区胞嘧啶-磷酸-鸟嘌呤二核苷酸岛(CGI),用甲基化实时荧光定量PCR技术检测其在87例卵巢癌和42例卵巢良性病变患者病变组织中的甲基化状态.结果 182个过甲基化位点和64个低甲基化位点(阳性率为25%以上的位点分别有18个和31个)组成了卵巢癌的DNA异常甲基化模式.87例卵巢癌和42例卵巢良性病变患者组织DNA中,基因LSM2、EGFLAM和CDKN2A的甲基化率依次为11%(10/87)和33%(14/42)、8%(7/87)和21%(9/42)、9%(8/87)和31%(13/42),与卵巢良性病变相比,卵巢癌中3个基因甲基化率均有显著下降,分别比较,差异均有统计学意义(P＜0.05).结论 建立卵巢癌的DNA异常甲基化模式是卵巢癌研究中非常重要的基础环节.基因EGFLAM、CDKN2A和LSM2启动子区CGI有可能成为新的卵巢癌特异性的低甲基化肿瘤标志物.
目的 建立卵巢上皮性癌(卵巢癌)的DNA異常甲基化模式,探討其在尋找新的卵巢癌特異性標誌物中的應用價值.方法 用激光顯微切割技術從20例卵巢癌組織冰凍切片中穫取的腫瘤細胞作為實驗對象,用原代培養的5例正常卵巢上皮細胞作為對照,用基于芯片技術的差異甲基化雜交(DMH)方法檢測卵巢癌的DNA異常甲基化模式.選擇7箇DMH結果顯示在卵巢癌中低甲基化的基因啟動子區胞嘧啶-燐痠-鳥嘌呤二覈苷痠島(CGI),用甲基化實時熒光定量PCR技術檢測其在87例卵巢癌和42例卵巢良性病變患者病變組織中的甲基化狀態.結果 182箇過甲基化位點和64箇低甲基化位點(暘性率為25%以上的位點分彆有18箇和31箇)組成瞭卵巢癌的DNA異常甲基化模式.87例卵巢癌和42例卵巢良性病變患者組織DNA中,基因LSM2、EGFLAM和CDKN2A的甲基化率依次為11%(10/87)和33%(14/42)、8%(7/87)和21%(9/42)、9%(8/87)和31%(13/42),與卵巢良性病變相比,卵巢癌中3箇基因甲基化率均有顯著下降,分彆比較,差異均有統計學意義(P＜0.05).結論 建立卵巢癌的DNA異常甲基化模式是卵巢癌研究中非常重要的基礎環節.基因EGFLAM、CDKN2A和LSM2啟動子區CGI有可能成為新的卵巢癌特異性的低甲基化腫瘤標誌物.
목적 건립란소상피성암(란소암)적DNA이상갑기화모식,탐토기재심조신적란소암특이성표지물중적응용개치.방법 용격광현미절할기술종20례란소암조직빙동절편중획취적종류세포작위실험대상,용원대배양적5례정상란소상피세포작위대조,용기우심편기술적차이갑기화잡교(DMH)방법검측란소암적DNA이상갑기화모식.선택7개DMH결과현시재란소암중저갑기화적기인계동자구포밀정-린산-조표령이핵감산도(CGI),용갑기화실시형광정량PCR기술검측기재87례란소암화42례란소량성병변환자병변조직중적갑기화상태.결과 182개과갑기화위점화64개저갑기화위점(양성솔위25%이상적위점분별유18개화31개)조성료란소암적DNA이상갑기화모식.87례란소암화42례란소량성병변환자조직DNA중,기인LSM2、EGFLAM화CDKN2A적갑기화솔의차위11%(10/87)화33%(14/42)、8%(7/87)화21%(9/42)、9%(8/87)화31%(13/42),여란소량성병변상비,란소암중3개기인갑기화솔균유현저하강,분별비교,차이균유통계학의의(P＜0.05).결론 건립란소암적DNA이상갑기화모식시란소암연구중비상중요적기출배절.기인EGFLAM、CDKN2A화LSM2계동자구CGI유가능성위신적란소암특이성적저갑기화종류표지물.
Objective To profile methylation alterations of cytosine-phosphate-guanosine islands (CGI)in epithelial ovarian cancer and investigate its applications for finding new candidate tumor markers.Methods Cancer cells were obtained by lager microdissection from 20 tissues of frozen-preserved epithelial ovarian tumors.Primary cultured epithelial cells were isolated from 5 tissues of normal ovaries.Differential methylation hybridization(DMH)based on microarray assay Was conducted using DNA to construct the aberrant DNA methylation pattern of epithelial ovarian cancer.MethyLight was conducted to verify the methylation status of 7 hypomethylated promoter CGI detected by DMH in tumor tissues of 87 patients with epithelial ovarian cancer and 42 patients with benigh ovarian diseases.Results The aberrant DNA methylation pattem of epithelial ovarian cancer were included 182 hypermethylated loci and 64 hypomethylated loci,of which the positive loci located more than 25%arrays were 18 and 31,respectively.The methylation ratio of gene LSM2,EGFLAM and CDKN2A in tissue DNA of patients with epithelial ovarian cancer and benign ovarian diseases Was 11%(10/87)versus 33%(14/42),8%(7/87)versus 21%(9/42),9%(8/87)versus 31%(13/42),respectively,which Was significantly decreased in tissues DNA of ovarian cancer than that from benigh ovarian diseases(P＜0.05).Conclusions The aberrant DNA methylation pattern of epithelial ovarian cancer is important for finding new cancer related genes.The promoter CGI of gene ISM2,EGFIAM and CDKN2A may be Hovel candidate for ovarian cancerspecific hypomethylated tumor markers.
