中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2012年
27期
1886-1888
,共3页
李伟伟%王全楚%宋新文%申保生%耿晓林%侯丽娟
李偉偉%王全楚%宋新文%申保生%耿曉林%侯麗娟
리위위%왕전초%송신문%신보생%경효림%후려연
肝硬化%NF-κB%免疫组织化学
肝硬化%NF-κB%免疫組織化學
간경화%NF-κB%면역조직화학
Liver cirrhosis%NF-kappa B%Immunohistochemistry
目的 研究慢性乙型肝炎患者肝组织中核转录因子( NF-κB) p65与肝纤维化的关系.方法 选取2008年7月至2009年12月在解放军第一五三中心医院住院的慢性乙型肝炎患者60例,行肝穿刺活组织检查确定肝纤维化分期(S0～S4),免疫组化技术测定Ⅰ、Ⅲ型胶原和NF-κB p65,研究其相关性.结果 NF-κB p65的表达与肝纤维化分期(S)呈明显的正相关性,即S4＞S3＞S2＞S1(S0)(P＜0.01),且与Ⅰ、Ⅲ型胶原的表达呈正相关性(P＜0.01).结论 NF-κB p65的表达增加与肝纤维化的发生及发展有关,其机制可能与促进Ⅰ、Ⅲ型胶原等细胞外基质分泌有关.
目的 研究慢性乙型肝炎患者肝組織中覈轉錄因子( NF-κB) p65與肝纖維化的關繫.方法 選取2008年7月至2009年12月在解放軍第一五三中心醫院住院的慢性乙型肝炎患者60例,行肝穿刺活組織檢查確定肝纖維化分期(S0～S4),免疫組化技術測定Ⅰ、Ⅲ型膠原和NF-κB p65,研究其相關性.結果 NF-κB p65的錶達與肝纖維化分期(S)呈明顯的正相關性,即S4＞S3＞S2＞S1(S0)(P＜0.01),且與Ⅰ、Ⅲ型膠原的錶達呈正相關性(P＜0.01).結論 NF-κB p65的錶達增加與肝纖維化的髮生及髮展有關,其機製可能與促進Ⅰ、Ⅲ型膠原等細胞外基質分泌有關.
목적 연구만성을형간염환자간조직중핵전록인자( NF-κB) p65여간섬유화적관계.방법 선취2008년7월지2009년12월재해방군제일오삼중심의원주원적만성을형간염환자60례,행간천자활조직검사학정간섬유화분기(S0～S4),면역조화기술측정Ⅰ、Ⅲ형효원화NF-κB p65,연구기상관성.결과 NF-κB p65적표체여간섬유화분기(S)정명현적정상관성,즉S4＞S3＞S2＞S1(S0)(P＜0.01),차여Ⅰ、Ⅲ형효원적표체정정상관성(P＜0.01).결론 NF-κB p65적표체증가여간섬유화적발생급발전유관,기궤제가능여촉진Ⅰ、Ⅲ형효원등세포외기질분비유관.
Objective To explore the relationship between the expression of NF-κB p65 and hepatic fibrosis in chronic hepatitis B (CHB) patients.Methods Sixty CHB patients with hepatic fibrosis underwent liver biopsy to determine the stages of liver fibrosis ( S0 - S4).The distribution and expression of collagens Ⅰ,Ⅲ and NF-κB p65 in different stages of fibrosis in liver tissue were observed by immunohistochemistry and the results analyzed statistically.Results The expression of NF-κB p65 was positively correlated with the stage of hepatic fibrosis.That was S4 ＞ S3 ＞ S2 ＞ S1 (S0) ( P ＜ 0.01 ).And it was also positively correlated with the expression of collagens Ⅰ and Ⅲ ( P ＜ 0.01 ).Conclusion The elevated expression of NF-κB p65 is closely associated with the occurrence and development of hepatic fibrosis.Its mechanism is probably related with the increased secretion of collagens Ⅰ and Ⅲ after theactivation of hepatic stellate cell.
