CAJ | 학술논문

目的观察正常升主动脉、升主动脉瘤样扩张及Stanford A型夹层动脉瘤(TAAD)患者升主动脉血管组织中alpha-1抗胰蛋白酶表达的差异,探讨alpha-1抗胰蛋白酶对于维持升主动脉血管结构完整性中的作用.方法收集28例升主动脉血管组织标本,TAAD 14例,升主动脉瘤样扩张7例,心脏移植供体心脏升主动脉7例.采用组织学、逆转录-聚合酶链反应(RT-PCR)法及Western blot 法对标本组织中的alpha-1抗胰蛋白酶进行检测分析.结果在基因水平上,alpha-1抗胰蛋白酶在升主动脉瘤样扩张中表达最高,正常血管组织中表达次之,TAAD患者升主动脉中表达最低(2.192±0.133、1.213±0.156、0.672±0.101,P<0.05).在蛋白水平上,alpha-1抗胰蛋白酶在升主动脉瘤样扩张中表达明显升高,TAAD患者升主动脉中表达次之,正常血管组织中最低(0.285±0.010、0.153±0.011、0.102±0.010,P<0.05).结论 alpha-1抗胰蛋白酶在正常人、升主动脉瘤样扩张及TAAD患者升主动脉血管组织均有表达,表达差异有统计学意义.alpha-1抗胰蛋白酶对于维持升主动脉血管结构的完整性具有潜在的保护作用.
목적 관찰정상승주동맥、승주동맥류양확장급Stanford A형협층동맥류(TAAD)환자승주동맥혈관조직중alpha-1항이단백매표체적차이,탐토alpha-1항이단백매대우유지승주동맥혈관결구완정성중적작용.방법 수집28례승주동맥혈관조직표본,TAAD 14례,승주동맥류양확장7례,심장이식공체심장승주동맥7례.채용조직학、역전록-취합매련반응(RT-PCR)법급Western blot 법대표본조직중적alpha-1항이단백매진행검측분석.결과 재기인수평상,alpha-1항이단백매재승주동맥류양확장중표체최고,정상혈관조직중표체차지,TAAD환자승주동맥중표체최저(2.192±0.133、1.213±0.156、0.672±0.101,P<0.05).재단백수평상,alpha-1항이단백매재승주동맥류양확장중표체명현승고,TAAD환자승주동맥중표체차지,정상혈관조직중최저(0.285±0.010、0.153±0.011、0.102±0.010,P<0.05).결론 alpha-1항이단백매재정상인、승주동맥류양확장급TAAD환자승주동맥혈관조직균유표체,표체차이유통계학의의.alpha-1항이단백매대우유지승주동맥혈관결구적완정성구유잠재적보호작용.
Objective To investigate whether alpha-1 antitrypsin is present in normal human aorta, ascending aortic aneurysm and stanford A type aortic dissection (TAAD) tissues and whether there are some expression differences in alpha-1 antitrypsin levels among them, and discuss its potential protective effects on aortic wall structure. Methods Histological method, semi-quantified reverse transcription polymerase chain reaction (RT-PCR) and Western blotting were used to test whether alpha-1 antitrypsin was present in normal human aorta, ascending aortic aneurysm and TAAD tissues. TAAD tissues in 14 cases, ascending aortic aneurysm tissues in 7 cases and normal ascending aorta tissues in 7 cases were harvested. Results alpha-1 antitrypsin expression was strongly increased in TAAD and ascending aortic aneurysm tissues as compared with normal aorta (0. 285 ±0. 010, 0. 153 ±0. 011,0. 102 ±0. 010,P <0. 05). alpha-1 antitrypsin was increased more significantly in ascending aortic aneurysm than in TAAD (0. 285 ±0. 010 vs 0. 153 ±0. 011 ,P < 0. 05 ). RT-PCR revealed that there was endogenous alpha-1 antitrypsin produced in aortic wall, and mRNA expression levels of them were highest in ascending aortic aneurym and lowest in TAAD (2. 192 ±0. 133, 1. 213 ±0. 156, 0. 672 ±0. 101 ,P <0. 05). Conclusion alpha-1 antitrypsin is present in normal human aorta, ascending aortic aneurysm and TAAD tissues, and there are differential expression differences among them. alpha-1 antitrypsin presents a potential pathway for protection of aortic wall structure.