CAJ | 학술논문

对巴西盾柱木(Peltophorum dubium)种子进行了4种处理的吸胀实验,即将种子分别置于10℃和27℃下0.2% Captan溶液中和-1.0MPa的PEG6000溶液中,每个处理有四个重复,40粒种子,置于培养皿中浸满实验溶液的双层过滤纸上,覆以透气良好的薄膜.种子在实验前均用98%的浓硫酸处理15分钟以打破机械休眠.吸胀曲线显示,种子在吸胀过程中最终重量的增加值为70%-150%,以后进入一个停滞期.在5种处理下(对照组,PEG6000溶液中10℃和27℃下预处理,Captan溶液中10℃和27℃下预处理),对种子萌发所受到的影响进行了实验.每一个处理分别进行3个次级处理,即在实验前,将种子用蒸馏水分别浸泡12、24和36小时.萌发率最高的是经过蒸馏水浸泡12小时对照组种子和PEG 27℃组的种子,萌发率达到100%;萌发率最低的是蒸馏水中浸泡36小时的PEG 27℃组的种子,萌发率为52%;经过蒸馏水浸泡24小时的PEG 10℃组种子的平均萌发时间为1.08天.蒸馏水中浸泡12小时的PEG 27℃组种子平均萌发时间为2.42天,其它处理的萌发时间值介于两者之间.对预处理和未经预处理种子通过加速老化实验,对其活力和生存力进行了测试.经72小时加速老化,种子萌发率低或没有萌发力.对照组种子在培养皿(27℃)和室温条件下蛭石中的萌发率都比处理组高,表现了对老化更强的抗性.
대파서순주목(Peltophorum dubium)충자진행료4충처리적흡창실험,즉장충자분별치우10℃화27℃하0.2% Captan용액중화-1.0MPa적PEG6000용액중,매개처리유사개중복,40립충자,치우배양명중침만실험용액적쌍층과려지상,복이투기량호적박막.충자재실험전균용98%적농류산처리15분종이타파궤계휴면.흡창곡선현시,충자재흡창과정중최종중량적증가치위70%-150%,이후진입일개정체기.재5충처리하(대조조,PEG6000용액중10℃화27℃하예처리,Captan용액중10℃화27℃하예처리),대충자맹발소수도적영향진행료실험.매일개처리분별진행3개차급처리,즉재실험전,장충자용증류수분별침포12、24화36소시.맹발솔최고적시경과증류수침포12소시대조조충자화PEG 27℃조적충자,맹발솔체도100%;맹발솔최저적시증류수중침포36소시적PEG 27℃조적충자,맹발솔위52%;경과증류수침포24소시적PEG 10℃조충자적평균맹발시간위1.08천.증류수중침포12소시적PEG 27℃조충자평균맹발시간위2.42천,기타처리적맹발시간치개우량자지간.대예처리화미경예처리충자통과가속노화실험,대기활력화생존력진행료측시.경72소시가속노화,충자맹발솔저혹몰유맹발력.대조조충자재배양명(27℃)화실온조건하질석중적맹발솔도비처리조고,표현료대노화경강적항성.
Peltophorum dubium seeds were set to imbibe with four treatments, soaked with solution Captan 0.2% under 10 and 27 oC, PEG 6000 -1.0 MPa under 10 and 27 oC. For each treatment there were four replicates with 40 seeds incubated in 9-cm Petri dishes with double filter paper moistened with testing solution. The imbibition curves showed that the final weight increase were from 70% to 150% in the treatments when imbibition entered a lag phase. Seeds were tested for effects on germination of five treatments: control group (nonprimed), primed with PEG6000 -1.0 MPa at 10 and 27 oC, primed with Captan 0.2% at 10 and 27 oC. For each treatment, there were three sub-treatments: seeds were soaked in distilled water for 12, 24 and 36h before the energy test. Germination percentages of nonprimed seeds and primed in PEG 27 oC soaked in distilled water during 12 h were the highest, reaching 100%. The lowest germination percentage occurred primed seeds with PEG6000 27 oC and soaked in distilled water during 36 h, which was only 52%. Germination mean time of primed seeds in PEG at 10 oC, soaked 24 h was 1.08 days, mean time of primed seeds in PEG at 27 oC soaked 12 h was 2.42 days. Accelerated ageing results showed low or no germination after ageing 72 h. Control group had a higher germination percentage and seeds were more resistant to deterioration than those in primed groups, both in Petri dish (27 oC) and vermiculate (room temperature).