CAJ | 학술논문

本文建立了一种可靠性高、重现性好的高效液相色谱(HPLC)测定山楂叶中熊果酸含量的方法,在测定中采用富集和固相萃取组合纯化工艺去除干扰物质.高效液相色谱测定条件为Hypersil(ODS)色谱柱,流动相为甲醇:0.2%磷酸二氢钠(90∶10,V/V),检测波长210 nm,流速0.8 mL/min.熊果酸浓度在100～800 μg/mL与峰面积存在良好线性关系(r2=0.9992),该方法准确可靠,日内稳定性标准偏差在0.6%～1.5%,日间稳定性标准偏差在0.7%～2.6%.为不同产地山楂叶中熊果酸含量建立有效的分析方法.
본문건립료일충가고성고、중현성호적고효액상색보(HPLC)측정산사협중웅과산함량적방법,재측정중채용부집화고상췌취조합순화공예거제간우물질.고효액상색보측정조건위Hypersil(ODS)색보주,류동상위갑순:0.2%린산이경납(90∶10,V/V),검측파장210 nm,류속0.8 mL/min.웅과산농도재100～800 μg/mL여봉면적존재량호선성관계(r2=0.9992),해방법준학가고,일내은정성표준편차재0.6%～1.5%,일간은정성표준편차재0.7%～2.6%.위불동산지산사협중웅과산함량건립유효적분석방법.
Quantitative HPLC analysis of ursolic acid in leaves of Hawthorn was established. The HPLC system used an Hypersil(ODS) column eluted with methanol:0.2% sodium dihydrogen phosphate aqueous = 90: 10,V/V) as solvents at a flow rate 0.8 mL/min and detected at 210 nm. Linear correlation ( r2 =0.9992) of the peak area and the concentration of ursolic acid over the concentration range 100 ～ 800 μg/mL was obtained. The method was precise with relative standard deviations for ursolic acid ranged between 0.6% ～ 1.5 % ( intraday ) and 0.7% ～ 2.6% ( interday ). The content of ursolic acid in the leaves of Hawthorn growing at different areas was determined to establish the effectiveness of the method.