云南植物研究
雲南植物研究
운남식물연구
ACTA BOTANICA YUNNANICA
2009年
5期
427-432
,共6页
AtWRKY2%转录调控因子%NaCl%甘露醇%渗透胁迫
AtWRKY2%轉錄調控因子%NaCl%甘露醇%滲透脅迫
AtWRKY2%전록조공인자%NaCl%감로순%삼투협박
AtWRKY2%Transcription factor%Nad%Mannitol%Osmotic stress
拟南芥WRKY2蛋白定位于细胞核,表明WRKY2是转录调控因子.WRKY2在不同器官组织中的表达分析显示在叶的表达量是最高的.在各种逆境条件下的表达分析显示:WRKY2的表达受NaCl和甘露醇比较强的诱导;KCl、LiCl、CaCl2和NaH2PO4均不诱导WRKY2的表达;ABA处理基本上不影响WRKY2基因的表达;另外,WRKY2的表达也不受病原菌、冷害和高温的诱导.这些结果表明WRKY2可能在NaCl和甘露醇引起的渗透胁迫反应中起一定的作用.
擬南芥WRKY2蛋白定位于細胞覈,錶明WRKY2是轉錄調控因子.WRKY2在不同器官組織中的錶達分析顯示在葉的錶達量是最高的.在各種逆境條件下的錶達分析顯示:WRKY2的錶達受NaCl和甘露醇比較彊的誘導;KCl、LiCl、CaCl2和NaH2PO4均不誘導WRKY2的錶達;ABA處理基本上不影響WRKY2基因的錶達;另外,WRKY2的錶達也不受病原菌、冷害和高溫的誘導.這些結果錶明WRKY2可能在NaCl和甘露醇引起的滲透脅迫反應中起一定的作用.
의남개WRKY2단백정위우세포핵,표명WRKY2시전록조공인자.WRKY2재불동기관조직중적표체분석현시재협적표체량시최고적.재각충역경조건하적표체분석현시:WRKY2적표체수NaCl화감로순비교강적유도;KCl、LiCl、CaCl2화NaH2PO4균불유도WRKY2적표체;ABA처리기본상불영향WRKY2기인적표체;령외,WRKY2적표체야불수병원균、냉해화고온적유도.저사결과표명WRKY2가능재NaCl화감로순인기적삼투협박반응중기일정적작용.
The fact that Arabidopsis WRKY2 protein was nuclear-localized supported that WRKY2 functioned as a transcription factor. The expression levels of WRKY2 in leaves were the highest in all tissues. The expression profile indicated that the expression levels of WRKY2 were elevated by Nad and mannitol treatments, while which were not induced by other treatments including KC1, LiCl, CaCl_2, NaH_2PO_4 , pathogenic germs, cold, heat and ABA. These results suggested that WRKY2 might be involved in osmotic stress induced by NaCl and mannitol.
