CAJ | 학술논문

目的探讨卡介菌多糖核酸(PNCG)治疗特应性皮炎(AD)前后,患者外周血人皮肤淋巴细胞相关抗原(CLA)阳性的T淋巴细胞表达不同细胞因子的频数及其与疾病严重程度的相关性.方法以双盲、随机、交叉对照方法应用卡介菌多糖核酸及安慰剂治疗AD患者,应用流式细胞术检测治疗前后AD患者外周血CLA~+T淋巴细胞表达不同细胞质内细胞因子(包括IL-4、IL-5、IFN-γ、TNF-α)的频数;利用AD皮损面积严重度指数积分(ADASIS)法,获取治疗前后AD患者病情严重程度积分.结果与安慰剂相比,卡介菌多糖核酸治疗后AD患者外周血中CLA~+T淋巴细胞内表达IL-5的频数明显下降;CLA~+T淋巴细胞内表达IFN-γ明显升高;卡介菌多糖核酸治疗后患者ADASIS积分下降.结论卡介菌多糖核酸作为一种免疫调节药物,可通过纠正CLA~+T淋巴细胞亚群的免疫失衡状态治疗特应性皮炎.
목적 탐토잡개균다당핵산(PNCG)치료특응성피염(AD)전후,환자외주혈인피부림파세포상관항원(CLA)양성적T림파세포표체불동세포인자적빈수급기여질병엄중정도적상관성.방법 이쌍맹、수궤、교차대조방법응용잡개균다당핵산급안위제치료AD환자,응용류식세포술검측치료전후AD환자외주혈CLA~+T림파세포표체불동세포질내세포인자(포괄IL-4、IL-5、IFN-γ、TNF-α)적빈수;이용AD피손면적엄중도지수적분(ADASIS)법,획취치료전후AD환자병정엄중정도적분.결과 여안위제상비,잡개균다당핵산치료후AD환자외주혈중CLA~+T림파세포내표체IL-5적빈수명현하강;CLA~+T림파세포내표체IFN-γ명현승고;잡개균다당핵산치료후환자ADASIS적분하강.결론 잡개균다당핵산작위일충면역조절약물,가통과규정CLA~+T림파세포아군적면역실형상태치료특응성피염.
Objective To investigate the influence of polysaccharide and nucleoside extract from bacille Calmett-Gueiin (PNCG) on the frequency of peripheral cutaneous lymphocyte-associated antigen positive (CLA~+) T lymphocyte derived lymphokines in atopic dermatitis (AD) and to explore their associations with the disease severity. Methods A randomized,double blind and placebo cross-over control method was used to treat the AD patients. Before and after the treatment,flow cytometry was used to measure the frequencies of IL-4,IL-5, IFN-γ,TNFα positive CLA~+T cells. Disease severity was evaluated by atopic dermatitis area and severity index score (ADASIS). Results After the PNCG treatment,the frequency of IL-5~+ CLA~+T cell decreased significantly,while IFN-γ increased,compared with the placebo con-trol group. PNCG could improve the ADASIS. Conclusion PNCG may treat AD through restoring the immune balance of CLA~+T cells.