CAJ | 학술논문

目的:采用TLC、HPLC和紫外分光光度法(UV)研究栀子黄OD值(栀子黄中栀子苷的最大吸光度A_(238nm)与西红花苷的最大吸光度A_(440nm)的比值)与栀了苷含量的关系.方法:采用硅胶柱层析从栀子中分离西红花苷纯品,然后分别采用TLC、HPLC和UV来定性和定量分析栀子乙醇提取部位和经大孔吸附树脂精制的栀子黄,并计算波长238nm和440nm处的卣分比吸光系数及OD值.结果:经过对以上数据的分析,发现西红花苷本身在波长238nm处有明显紫外吸收,采用OD值来控制栀子黄中的栀子苷将高估栀子苷含量.结论:栀子黄中栀子苷的控制不宜采用OD值作为评价指标,而应采用具有分离功能的高效液相色谱法来作为质量控制的手段.
목적:채용TLC、HPLC화자외분광광도법(UV)연구치자황OD치(치자황중치자감적최대흡광도A_(238nm)여서홍화감적최대흡광도A_(440nm)적비치)여치료감함량적관계.방법:채용규효주층석종치자중분리서홍화감순품,연후분별채용TLC、HPLC화UV래정성화정량분석치자을순제취부위화경대공흡부수지정제적치자황,병계산파장238nm화440nm처적유분비흡광계수급OD치.결과:경과대이상수거적분석,발현서홍화감본신재파장238nm처유명현자외흡수,채용OD치래공제치자황중적치자감장고고치자감함량.결론:치자황중치자감적공제불의채용OD치작위평개지표,이응채용구유분리공능적고효액상색보법래작위질량공제적수단.
TLC.HPLC and UV techniques were used to investigate the relationship between OD value(defined as the ratio of maximum absorbance value at 238 nm wavelength of genipin to maximum absorbance value at 410 nm wavelength of crocins)of gardenia yellow and genipin and crocins contents in it.Compounds with high purity were isolated from gardenia yellow by gel column chromatography and then subjected to qualitative and quantitative analysis by TLC,HPLC and UV.In addition,E_(1cm 238)~(1%),E_(1cm440)~(1%) and OD values were calculated.UV spectral data clearly indicated strong absorption of crocins at 238 nm wavelength.As a result,OD value might cause an over-estimatation of genipin content in gardenia yellow when used for quantitation.Therefore,it is inaccurate to represent genipin content in gardenia yellow by OD value.Moreover,this study implies that HPLC is a scientific and proper tool for analyzing genipin in natural pigments.