CAJ | 학술논문

目的瞬时受体势(TRP)是一种新发现的钙通道,是当前细胞信号转导领域内的研究热点之一.以往有关TRP通道的研究主要集中在神经系统,现已开始向其它研究领域扩展.笔者推测,TRP通道可能与肝癌细胞的增殖有关.该研究将证实此推测.方法应用TRPC通道抑制剂(SKF-96365)和RNAi技术抑制该通道蛋白在肝癌细胞中的表达和功能,观察TRPC被抑制前后肝癌细胞体外增殖能力的改变.结果研究发现,应用SKF-96365和siRNA方法阻滞TRPC6的通道功能后,肝癌细胞(HepG2和SMMC-7721)的增殖被明显抑制.结论该研究证明TRPC6通道介导了肝癌细胞的增殖,为肝癌细胞增殖机制的研究提供了新的思路,探索了肝癌临床治疗新的细胞膜上的靶点,同时也拓展了TRP通道的研究领域.
목적 순시수체세(TRP)시일충신발현적개통도,시당전세포신호전도영역내적연구열점지일.이왕유관TRP통도적연구주요집중재신경계통,현이개시향기타연구영역확전.필자추측,TRP통도가능여간암세포적증식유관.해연구장증실차추측.방법 응용TRPC통도억제제(SKF-96365)화RNAi기술억제해통도단백재간암세포중적표체화공능,관찰TRPC피억제전후간암세포체외증식능력적개변.결과 연구발현,응용SKF-96365화siRNA방법조체TRPC6적통도공능후,간암세포(HepG2화SMMC-7721)적증식피명현억제.결론 해연구증명TRPC6통도개도료간암세포적증식,위간암세포증식궤제적연구제공료신적사로,탐색료간암림상치료신적세포막상적파점,동시야탁전료TRP통도적연구영역.
Objective Transient recepter potential (TRP) is a kind of novel Ca2+ -permeant channel and one of the hot topics in the current basic researches. The researches on TRP were mostly focused on nervous system in the past, and now they come to extend to other areas. We hypothesized that TRP channels should be likely related to cell proliferation of bepatocellular carcinoma(HCC). This study was designed to confirm this hypothesis. Methods After inhibiting the function of TRPC channels by pharmacological means (SKF-96365) and RNAi, we observed the effect of this inhibition on cell proliferation in vitro. Results After inhibition of TRP channels by SKF-96365 and RNAi, the proliferation of two HCC cell lines of HepG2 and SMMC-7721 was significantly repressed. Conclusion The TRPC6 channels are implicated in HCC cell proliferation. This study not only provides new insights into proliferation of HCC cells and subsequent cancer intervention strategies but also opens a new area for TRP channel study.