CAJ | 학술논문

目的观察白藜芦醇对活化的内皮细胞E-选择素和巨噬细胞趋化蛋白-1表达的影响.方法白藜芦醇预孵育人脐静脉内皮细胞后,将培养的人脐静脉内皮细胞随机分为3组,分别为对照组、肿瘤坏死因子(TNF)组和白藜芦醇+TNF-α组,用TNF-α(10μg/L)刺激,流式细胞术检测内皮细胞E-选择素分子的表达,半定最反转录聚合酶链反应检测E-选择素和巨噬细胞趋化蛋白-1mRNA的表达. 结果 TNF-α可诱导内皮细胞E-选择素和巨噬细胞趋化蛋白-1表达,白藜芦醇(10 μmol/L)能够抑制E_选择素的表达,对照组、TNF组和白藜芦醇+TNF组E-选择素阳性细胞分别为(3.7±1.6)%、(47.8±3.2)%和(15.3±1.7)%,两两比较差异有统计学意义(P<0.05或P<0.01);并浓度依赖性地抑制E-选择素和巨噬细胞趋化蛋白-1基因的转录. 结论白藜芦醇可能通过抑制内皮细胞E-选择素和巨噬细胞趋化蛋白-1基因表达发挥抗动脉粥样硬化作用.
목적 관찰백려호순대활화적내피세포E-선택소화거서세포추화단백-1표체적영향.방법 백려호순예부육인제정맥내피세포후,장배양적인제정맥내피세포수궤분위3조,분별위대조조、종류배사인자(TNF)조화백려호순+TNF-α조,용TNF-α(10μg/L)자격,류식세포술검측내피세포E-선택소분자적표체,반정최반전록취합매련반응검측E-선택소화거서세포추화단백-1mRNA적표체. 결과 TNF-α가유도내피세포E-선택소화거서세포추화단백-1표체,백려호순(10 μmol/L)능구억제E_선택소적표체,대조조、TNF조화백려호순+TNF조E-선택소양성세포분별위(3.7±1.6)%、(47.8±3.2)%화(15.3±1.7)%,량량비교차이유통계학의의(P<0.05혹P<0.01);병농도의뢰성지억제E-선택소화거서세포추화단백-1기인적전록. 결론 백려호순가능통과억제내피세포E-선택소화거서세포추화단백-1기인표체발휘항동맥죽양경화작용.
Objective To investigate the effect of resveratrol on the expressions of E-selectin and monocyte chemoattractant protein-1 (MCP-1) in activated endothelial cells. Methods After being pretreated with resveratrol followed by tumor necrosis factor-α (TNF-α) stimulation, human umbilical vein endothelial cells (HUVEC) were randomly divided into three groups: TNF group,resveratrol+TNF-α group and control group. The expression of E-selectin molecule on the surface of HUVEC was detected by flow cytometric analysis and the mRNA expressions of E-selectin and MCP -1 were determined by semiquantitative RT-PCR. Results TNF-α induced the expression of E-selectin and MCP-I of HUVEC. Resveratrol (10 μmol/L) inhibited E-selectin expression. The positive cells of E-selectin in TNF group, resveratrol + TNF-α group and control group were(47.84±3.2)%, (15.3±1.7)% and (3.74±1.6)%, respectively, and the differences among the three groups were statistically significant (P<0.05). Conclusions Resveratrol may contribute to the anti-atherosclerotic effect by inhibiting the expression of E-seleetin and MCP-1 of HUVEC.