CAJ | 학술논문

目的观察大麻素受体1( CB1)和受体2(CB2)在肝纤维化模型C57小鼠肝组织表达及意义.方法 30只C57小鼠被随机分为3组,即正常对照组、模型对照组和模型秋水仙碱组,每组10只.采用四氯化碳( CCl4)腹腔注射制造小鼠肝纤维化模型,通过免疫组织化学染色方法检测肝组织中CB1和CB2的表达,同时进行肝组织炎症(G)评分和纤维化(S)评分.结果模型对照组、模型秋水仙碱组及正常对照组肝组织G评分及S评分比较,差异有统计学意义(F=125.41,P=0.00；F=99.18,P=0.00),且模型对照组肝组织G评分及S评分均显著高于模型秋水仙碱组,差异均有统计学意义(P均＜0.01).模型对照组、模型秋水仙碱组肝组织及正常对照组CB1和CB2评分比较,差异有统计学意义(F=29.27,P=0.00；F=36.99,P=0.00),且模型对照组肝组织CB1和CB2评分均显著高于模型秋水仙碱组,差异具有统计学意义(P＜0.05或P＜0.01).模型对照组和模型秋水仙碱组中CB1、CB2、G和S评分四者之间均具有相关性(P均＜0.05),且随着G、S评分的不断增加,CB1和CB2评分逐渐增加.结论 CB1和CB2在肝纤维化模型C57小鼠肝组织中表达均明显增强,与肝组织炎症和肝纤维化程度有显著相关性.
목적 관찰대마소수체1( CB1)화수체2(CB2)재간섬유화모형C57소서간조직표체급의의.방법 30지C57소서피수궤분위3조,즉정상대조조、모형대조조화모형추수선감조,매조10지.채용사록화탄( CCl4)복강주사제조소서간섬유화모형,통과면역조직화학염색방법검측간조직중CB1화CB2적표체,동시진행간조직염증(G)평분화섬유화(S)평분.결과 모형대조조、모형추수선감조급정상대조조간조직G평분급S평분비교,차이유통계학의의(F=125.41,P=0.00；F=99.18,P=0.00),차모형대조조간조직G평분급S평분균현저고우모형추수선감조,차이균유통계학의의(P균＜0.01).모형대조조、모형추수선감조간조직급정상대조조CB1화CB2평분비교,차이유통계학의의(F=29.27,P=0.00；F=36.99,P=0.00),차모형대조조간조직CB1화CB2평분균현저고우모형추수선감조,차이구유통계학의의(P＜0.05혹P＜0.01).모형대조조화모형추수선감조중CB1、CB2、G화S평분사자지간균구유상관성(P균＜0.05),차수착G、S평분적불단증가,CB1화CB2평분축점증가.결론 CB1화CB2재간섬유화모형C57소서간조직중표체균명현증강,여간조직염증화간섬유화정도유현저상관성.
Objective To investigate hepatic expressions and significances of cannabinoid receptor 1 (CB1) and cannabinoid receptor 2(CB2) in C57 mice with experimental cirrhosis.Methods Thirty C57 mice were randomly divided into three groups,i.e.normal control group,model control group and model colchicine group.Hepatic fibrosis model was prepared by intraperitoneal injection of carbon tetrachloride.The expressions of CB1 and CB2 in liver tissue of mice were observed by immunohistochemistry.The scores of inflammation grade (G) and fibrosis stage (S) were simultaneously performed.Results The scores of G and S in model control group and model colchicine group were significantly higher than those in normal control group( F =125.41,P =0.00; F =99.18,P =0.00).The scores of G and S in model control group were significantly higher than those in model colchicine group(P ＜0.01 ).The scores of CB1 and CB2 expressions in model control group and model colchicine group were significantly higher than those in normal control group ( F =29.27,P =0.00; F =36.99,P =0.00).The scores of CB1 and CB2 in model control group were significantly higher than those in model colchicine group( P ＜ 0.05 or P ＜ 0.01 ).There were significant relationships among scores of CB1,CB2,G and S in model control group and model colchicine group(Ps ＜0.05).As the scores of G and S became higher,the expressions of CB1 and CB2 gradually became more intensive.Conclusion The hepatic expressions of CB1 and CB2 in C57 mice with experimental cirrhosis increased significantly and have significant relationship with the grades of liver tissue inflammation and fibrosis.