CAJ | 학술논문

目的探讨葡萄籽原花青素(GSPE)对糖尿病大鼠心肌糖基化终末产物受体(RAGE)、核转录因子κB(NF-κB)和结缔组织生长因子(CTGF)的影响.方法将链脲佐菌素诱导的糖尿病大鼠30只随机分为两组,糖尿病未治疗组(糖尿病1组)和糖尿病GSPE治疗组(糖尿病2组,每日给予GSPE 250 mg/kg灌胃)各15只；正常大鼠20只随机分为正常对照组(对照l组)和正常GSPE治疗组(对照2组,每日给予GSPE 250 mg/kg灌胃)各10只,24周后采血检测空腹血糖(FBG)、糖基化终末产物(AGEs),免疫组织化学染色和Western blot测定心肌NF-κB蛋白的表达,并应用Westernblot测定各组心肌RAGE和CTGF的蛋白表达变化.结果糖尿病1组FBG、血清AGEs含量较对照1组显著升高(P＜0.05),经GSPE治疗后,血清AGEs含量显著降低(P＜0.05),而FBG降低差异无统计学意义；糖尿病1组心肌组织RAGE、NF-κB和CTGF蛋白表达较对照1组显著升高(P＜0.05),GSPE能够显著抑制RAGE、NF-κB和CTGF蛋白表达.结论 GSPE对糖尿病心肌病具有保护作用,其可能机制与抑制糖尿病大鼠AGEs-RAGE、NF-κB和CTGF的表达有关.
목적 탐토포도자원화청소(GSPE)대당뇨병대서심기당기화종말산물수체(RAGE)、핵전록인자κB(NF-κB)화결체조직생장인자(CTGF)적영향.방법 장련뇨좌균소유도적당뇨병대서30지수궤분위량조,당뇨병미치료조(당뇨병1조)화당뇨병GSPE치료조(당뇨병2조,매일급여GSPE 250 mg/kg관위)각15지；정상대서20지수궤분위정상대조조(대조l조)화정상GSPE치료조(대조2조,매일급여GSPE 250 mg/kg관위)각10지,24주후채혈검측공복혈당(FBG)、당기화종말산물(AGEs),면역조직화학염색화Western blot측정심기NF-κB단백적표체,병응용Westernblot측정각조심기RAGE화CTGF적단백표체변화.결과 당뇨병1조FBG、혈청AGEs함량교대조1조현저승고(P＜0.05),경GSPE치료후,혈청AGEs함량현저강저(P＜0.05),이FBG강저차이무통계학의의；당뇨병1조심기조직RAGE、NF-κB화CTGF단백표체교대조1조현저승고(P＜0.05),GSPE능구현저억제RAGE、NF-κB화CTGF단백표체.결론 GSPE대당뇨병심기병구유보호작용,기가능궤제여억제당뇨병대서AGEs-RAGE、NF-κB화CTGF적표체유관.
Objective To investigate the effects of grape seed proanthocyanidin extracts (GSPE) on advanced glycation end product receptor (RAGE),NF-Κb and connective tissue growth factor (CTGF) in the myoeardium of diabetic rats.Methods Total 30 streptozotocin (STZ) induced diabetic rats were randomly divided into 2 groups:diabetic group (DM1,n-15) and GSPE (250 mg/ kg,i.g) treated diabetic group (DM2,n= 15).Another two control groups:normal rats(C1,n= 10)and normal rats treated with GSPE (250 mg/kg,i.g) (C2 group,n= 10) were also observed.After 24 weeks,blood was collected to measure fasting plasma glucose (FBG) and RAGE.The protein expression of NF-Κb was determined in myocardial tissue by immunohistochemical staining and Western blot.The protein expressions of RAGE and CTGF were measured by Western blot.Results The levels of FBG and RAGE were significantly higher in diabetic rats than in control rats (P＜0.05).After GSPE treatment,RAGE level significantly reduced (P＜0.05),but FBG had no change in diabetic rats.The protein expressions of RAGE,NF-Κb and CTGF in the myocardial tissue of diabetic rats had marked increase compared with control rats (P＜ 0.05),however,their levels significantly reduced after GSPE treatment (P＜0.05).Conclusions GSPE may protect diabetic rats against cardiomyopathy,possibly by decreasing the protein expressions of RAGE,NF-Κb and CTGF.