CAJ | 학술논문

目的观察食管癌患者外周血淋巴细胞热休克蛋白(HSP)27和HSP72的表达,探讨其与临床病理生理特征的关系.方法采用流式细胞术检测45例食管癌患者外周血淋巴细胞HSP27和HSFP72的表达水平,并以43例健康受试者作为对照.结果食管癌外周血淋巴细胞HSP27、HSP72的表达量分别为17.32±2.97和19.46±5.91,均低于对照组(P＜0.05),两者表达量呈正相关(P＜0.01),食管癌外周血淋巴细胞HSP27的表达量与临床病理生理特征无明显相关(P＞0.05),而HSP72的表达量与食管癌原发肿瘤的大小、淋巴结转移及临床分期相关(P＜0.05).结论食管癌外周血淋巴细胞HSP27、HSP72表达下调,HSP72与食管癌的发生发展及淋巴结转移相关.
목적 관찰식관암환자외주혈림파세포열휴극단백(HSP)27화HSP72적표체,탐토기여림상병리생리특정적관계.방법 채용류식세포술검측45례식관암환자외주혈림파세포HSP27화HSFP72적표체수평,병이43례건강수시자작위대조.결과 식관암외주혈림파세포HSP27、HSP72적표체량분별위17.32±2.97화19.46±5.91,균저우대조조(P＜0.05),량자표체량정정상관(P＜0.01),식관암외주혈림파세포HSP27적표체량여림상병리생리특정무명현상관(P＞0.05),이HSP72적표체량여식관암원발종류적대소、림파결전이급림상분기상관(P＜0.05).결론 식관암외주혈림파세포HSP27、HSP72표체하조,HSP72여식관암적발생발전급림파결전이상관.
Objective To investigate the relationship between the levels of heat shock protein (HSP) 27 and HSP72 in peripheral blood lymphocytes and clinical pathophysiological characteristics in patients with esophagus cancer. Methods Flow cytometry was used to detect the expression of HSP27 and HSP72 in peripheral blood lymphocytes of 45 patients with esophagus cancer and 43 healthy controls. Resu1ts The levels of HSP27 (17.32 ±2. 97) and HSP72 ( 19.46 ±5. 91 ) in the peripheral blood lymphocytes of patients with esophagus cancer were decreased as compared with those in normal controls ( P ＜0. 05 ). The correlation analysis revealed that HSP27 level was positively correlated to HSP72 level (P ＜0. 01 ). The Level of HSP27 in peripheral blood lymphocytes had no correlation to clinical pathophysiologicai characteristics of patients with esophagus cancer. The level of HSP72 in peripheral blood lymphocytes was related to tumor size, lymph node metastasis and clinical stages (P ＜0. 05), but not to sex, age andpathological types of esophagus cancer (P ＞ 0. 05). Conclusion The expression of HSP27 and HSP72 is decreased in peripheral blood lymphocytes of patients with esophagus cancer and HSP72 may play an important role in tumor growth and lymph node metastasis. The level of HSP72 in peripheral blood lymphocytes may be used as a predictor for prognosis.