CAJ | 학술논문

用液/液扩散法, 从分别含Cr和Mn的无氨培养基中生长的固氮菌(Azotobacter vinelandii Lipmann)突变种UW3中纯化出的CrFe蛋白和MnFe蛋白, 在合适的结晶条件下生长出深棕色大单晶(最大晶体的尺寸分别为0.20 mm×0.20 mm×0.07 mm 和 0.18 mm×0.18 mm×0.05 mm).PEG、MgCl2和NaCl 的浓度对这两种蛋白的出晶时间、晶核数目、晶体大小和形状都有明显影响.结果表明,用此结晶法有利于CrFe蛋白和MnFe蛋白生长出可供X-射线衍射分析的大单晶.
용액/액확산법, 종분별함Cr화Mn적무안배양기중생장적고담균(Azotobacter vinelandii Lipmann)돌변충UW3중순화출적CrFe단백화MnFe단백, 재합괄적결정조건하생장출심종색대단정(최대정체적척촌분별위0.20 mm×0.20 mm×0.07 mm 화 0.18 mm×0.18 mm×0.05 mm).PEG、MgCl2화NaCl 적농도대저량충단백적출정시간、정핵수목、정체대소화형상도유명현영향.결과표명,용차결정법유리우CrFe단백화MnFe단백생장출가공X-사선연사분석적대단정.
By using the liquid/liquid diffusion method at a suitable crystallization conditions, large single and dark brown crystals (the sides of the largest crystals were 0.20 mm×0.20 mm×0.07 mm and 0.18 mm×0.18 mm×0.05 mm, respectively) could be obtained from the solutions of nitrogenase CrFe protein and MnFe protein purified from a mutant UW3 of Azotobacter vinelandii Lipmann grown in Cr- or Mn-containing but NH3-free medium. The time of crystal formation, as well as the number, size, shape and quality of crystals obviously depended on the concentrations of PEG, MgCl2 and NaCl. The liquid/liquid diffusion method seems to benefit CrFe protein and MnFe protein for the growth of large single crystals for X-ray diffraction analysis.