CAJ | 학술논문

采用SSR分子标记技术对43个茶树品种(系)进行了遗传多态性分析.用37对可扩增引物对43个茶树品种(系)扩增,经8%非变性聚丙烯酰胺凝胶电泳检测,其中有34对引物产生多态性,不同引物扩增带数分布在1～11条之间,扩增片段大小介于150～350 bp.应用DPS软件,进行遗传距离和相似性系数计算,43份材料遗传距离的变化范围在0.059～0.820之间,说明供试茶树资源间的遗传变异十分宽广.根据UPGMA法构建聚类树状图,在平均遗传距离水平上,可将43份材料划分为7个类群.
채용SSR분자표기기술대43개다수품충(계)진행료유전다태성분석.용37대가확증인물대43개다수품충(계)확증,경8%비변성취병희선알응효전영검측,기중유34대인물산생다태성,불동인물확증대수분포재1～11조지간,확증편단대소개우150～350 bp.응용DPS연건,진행유전거리화상사성계수계산,43빈재료유전거리적변화범위재0.059～0.820지간,설명공시다수자원간적유전변이십분관엄.근거UPGMA법구건취류수상도,재평균유전거리수평상,가장43빈재료화분위7개류군.
SSR molecular marker were used to analyze the genetic polymorphism of tea germplasm.These 37 pairs of primer were amplified with 43 tea cultivars,and their PCR products were visualized by 8% native polyacrylamide gel electrophoresis.The polymorphic alleles were visualized from the PCR products amplified by 34 pairs of primer out of 37.The number of bands per primer pair ranged from 1 to 11 and the SSR fragment size of different SSR locus ranged from 150 to 350 bp.Based on the SSR results,the genetic distance and similarity coefficient were calculated using the Nei & Lei's coefficient method by DPS software.The results showed that the coefficient genetic distance among 43 accessions ranged from 0.059 to 0.820 indicating the gene differentiation was very remarkable among tested cultivars.Based on the genetic distance and the UPGMA cluster,all the 43 accessions were clustered to 7 groups at the average genetic distance level.