中南大学学报（医学版）
中南大學學報（醫學版）
중남대학학보（의학판）
JOURNAL OF CENTRAL SOUTH UNIVERSITY (MEDICAL SCIENCES)
2009年
7期
689-692,封3
,共5页
镇静催眠药%反向高效液相色谱法%血药浓度
鎮靜催眠藥%反嚮高效液相色譜法%血藥濃度
진정최면약%반향고효액상색보법%혈약농도
sedative hypnotics%reversed phase high-performance liquid chromatography%blood drug level
目的:建立人血浆中地西泮、硝西泮、奥沙西泮、艾司唑仑和阿普唑仑的反向高效液相色谱测定法(RP-HPLC).方法:以血浆为基质,配制含有5种镇静催眠药的混合标准品,取1 mL样品加入50 mg/L卡马西平10μL为内标,乙酸乙酯萃取后用100 μL流动相溶解,进样20 μL,通过高效液相色谱仪检测人血浆基质中5种镇静催眠药浓度.色谱条件:色谱柱为C18柱(4.6 mm×250 mm),柱温30℃,流动相为甲醇:水(65∶35),流速1.0 mL/min,紫外检测波长为230 nm.结果:地西泮、硝西泮、奥沙西沣、艾司唑仑和阿普唑仑在5～1 200μg/L范围内线性关系良好(r≥0.9966,P<0.05),加样回收率在95.5%～105.6%,萃取回收率均在75%以上,日内及日间变异相对标准偏差(RSD)均小于10%(n=5).结论:RP-HPLC方法简便、准确、灵敏,适用于人血浆中地西泮、硝西泮、奥沙西泮、艾司唑仑和阿普唑仑的血药浓度的同时检测.
目的:建立人血漿中地西泮、硝西泮、奧沙西泮、艾司唑崙和阿普唑崙的反嚮高效液相色譜測定法(RP-HPLC).方法:以血漿為基質,配製含有5種鎮靜催眠藥的混閤標準品,取1 mL樣品加入50 mg/L卡馬西平10μL為內標,乙痠乙酯萃取後用100 μL流動相溶解,進樣20 μL,通過高效液相色譜儀檢測人血漿基質中5種鎮靜催眠藥濃度.色譜條件:色譜柱為C18柱(4.6 mm×250 mm),柱溫30℃,流動相為甲醇:水(65∶35),流速1.0 mL/min,紫外檢測波長為230 nm.結果:地西泮、硝西泮、奧沙西灃、艾司唑崙和阿普唑崙在5～1 200μg/L範圍內線性關繫良好(r≥0.9966,P<0.05),加樣迴收率在95.5%～105.6%,萃取迴收率均在75%以上,日內及日間變異相對標準偏差(RSD)均小于10%(n=5).結論:RP-HPLC方法簡便、準確、靈敏,適用于人血漿中地西泮、硝西泮、奧沙西泮、艾司唑崙和阿普唑崙的血藥濃度的同時檢測.
목적:건립인혈장중지서반、초서반、오사서반、애사서륜화아보서륜적반향고효액상색보측정법(RP-HPLC).방법:이혈장위기질,배제함유5충진정최면약적혼합표준품,취1 mL양품가입50 mg/L잡마서평10μL위내표,을산을지췌취후용100 μL류동상용해,진양20 μL,통과고효액상색보의검측인혈장기질중5충진정최면약농도.색보조건:색보주위C18주(4.6 mm×250 mm),주온30℃,류동상위갑순:수(65∶35),류속1.0 mL/min,자외검측파장위230 nm.결과:지서반、초서반、오사서풍、애사서륜화아보서륜재5～1 200μg/L범위내선성관계량호(r≥0.9966,P<0.05),가양회수솔재95.5%～105.6%,췌취회수솔균재75%이상,일내급일간변이상대표준편차(RSD)균소우10%(n=5).결론:RP-HPLC방법간편、준학、령민,괄용우인혈장중지서반、초서반、오사서반、애사서륜화아보서륜적혈약농도적동시검측.
Objective To determine diazepam, nitrazepam, oxazepam, estazolam, and al-prazolam simultaneously in human plasma by reversed phase high-performance liquid chromatography (RP-HPLC). Methods Ten microliter carbamazepine (50 mg/L)as the internal standard was added into 1 mL sample, which contained the 5 mixed sedative hypnotics as standard substance and human plasma as ground substance. They were extracted with acetoacetate from plasma samples, and then were dissolved by 100 μL mobile phase. The blood drug levels were analyzed by high perform-ance liquid chromatograph with 20 μL sample injection on a chromatographic column C 18 (4.6 mm×250 mm)at 30℃. The mobile phase consisted of methanol and water (65:35) , and the flow rate was 1.0 mL/min. The ultraviolet detection wavelength was 230 nm. Results The linearity range of the 5 drugs was 5～1 200 μg/L(r≥0.9966, P<0.05). The recovery rate was 95.5%～105.6%. The extraction recovery rate was more than 75%. The relative standard deviation (RSD) of intra-day and inter-day was less than 10% (n=5). Conclusion RP-HPLC method is convert-ient, accurate and sensitive for simultaneous determination of the concentration of diazepam, nitraze-pam, oxazepam, estazolam, and alprazolam in human plasma.
