CAJ | 학술논문

基于盐酸曲马多对联吡啶钌(Ru(bpy)32+)的电化学发光信号有较强的增敏作用, 建立了一种多壁碳纳米管/壳聚糖-联吡啶钌复合物修饰的石墨电极上电化学发光检测盐酸曲马多的电化学发光新方法. 通过循环伏安扫描结果表明, 多壁碳纳米管表现出极好的电分析活性, 对联吡啶钌具有较好的电催化作用, 并可应用于盐酸曲马多药物的测定. 在最佳实验条件下, 测定曲马多浓度在6.0×10-4～5.0×10-6 mol/L与相对发光强度成线性关系(r=09982), 检出限(S/N=3)为2.0×10-6 mol/L. 连续平行测定曲马多溶液(5.0×11-5 mol/L) 8次, 发光强度的RSD为3.1%.
기우염산곡마다대련필정조(Ru(bpy)32+)적전화학발광신호유교강적증민작용, 건립료일충다벽탄납미관/각취당-련필정조복합물수식적석묵전겁상전화학발광검측염산곡마다적전화학발광신방법. 통과순배복안소묘결과표명, 다벽탄납미관표현출겁호적전분석활성, 대련필정조구유교호적전최화작용, 병가응용우염산곡마다약물적측정. 재최가실험조건하, 측정곡마다농도재6.0×10-4～5.0×10-6 mol/L여상대발광강도성선성관계(r=09982), 검출한(S/N=3)위2.0×10-6 mol/L. 련속평행측정곡마다용액(5.0×11-5 mol/L) 8차, 발광강도적RSD위3.1%.
Based on the increasing effect of tramadol on Ru(bpy)32+ electrochemiluminescence system, an electrochemiluminescence method for the determination of tramadol hydrochloride at a multi-wall carbon nanotube/chitosan-Ru(bpy)32+ composite film modified graphite electrode was described. Cyclic voltammetric results clearly showed that multi-wall carbon nanotubes possessed an excellent electrocatalytic activity, also could accelerate electron transfer rate between the redox-active Ru(bpy)32+ and the electrode. The results showed that the proposed method was sensitive for the determination of tramadol hydrochloride. Under the optimum conditions, the tramadol hydrochloride concentration in the range of 6.0×10-4～5.0×10-6 mol/L (r=0.9982) was proportional to the enhanced ECL signal, and the detection limit (defined as the concentration that could be detected at the signal-to-noise ratio of 3) for tramadol hydrochloride was 2.0×10-6 mol/L. The relative standard derivation was 3.1% (c=2.0×10-6 mol/L, n=8). The method has been used to determine the tramadol tablets with satisfactory results.