福建师范大学学报(自然科学版)
福建師範大學學報(自然科學版)
복건사범대학학보(자연과학판)
JOURNAL OF FUJIAN TEACHERS UNIVERSITY (NATURAL SCIENCE EDITION)
2009年
6期
71-76
,共6页
唐良华%苏敏%陈梦虹%吕博彦%蔡志雄
唐良華%囌敏%陳夢虹%呂博彥%蔡誌雄
당량화%소민%진몽홍%려박언%채지웅
均匀设计%脂肪酶%布洛芬%反应器%反应动力学
均勻設計%脂肪酶%佈洛芬%反應器%反應動力學
균균설계%지방매%포락분%반응기%반응동역학
uniform design%lipase%ibuprofen%reactor%kinetics
为了提高固定化扩展青霉TS414脂肪酶(PEL)对外消旋布洛芬的拆分效率, 利用均匀设计的实验方法,在酶反应器中考察和优化了布洛芬、固定化脂肪酶、1-丙醇等几个参数对拆分反应的影响. 优化后的实验结果表明:当反应体系中布洛芬为200 mg,固定化酶的加量为6 g, 1-丙醇为0.383 mL,反应达到平衡时,布洛芬拆分反应的底物转化率接近50%,底物的对映体过量值达到94.3%,产物的对映体过量值则稳定在98%以上.而且固定化PEL催化拆分反应的周期缩短至12 h.同时,基于乒乓反应机制的动力学方程,建立了相应的拆分反应动力学模型,并得到了该模型的有关参数.
為瞭提高固定化擴展青黴TS414脂肪酶(PEL)對外消鏇佈洛芬的拆分效率, 利用均勻設計的實驗方法,在酶反應器中攷察和優化瞭佈洛芬、固定化脂肪酶、1-丙醇等幾箇參數對拆分反應的影響. 優化後的實驗結果錶明:噹反應體繫中佈洛芬為200 mg,固定化酶的加量為6 g, 1-丙醇為0.383 mL,反應達到平衡時,佈洛芬拆分反應的底物轉化率接近50%,底物的對映體過量值達到94.3%,產物的對映體過量值則穩定在98%以上.而且固定化PEL催化拆分反應的週期縮短至12 h.同時,基于乒乓反應機製的動力學方程,建立瞭相應的拆分反應動力學模型,併得到瞭該模型的有關參數.
위료제고고정화확전청매TS414지방매(PEL)대외소선포락분적탁분효솔, 이용균균설계적실험방법,재매반응기중고찰화우화료포락분、고정화지방매、1-병순등궤개삼수대탁분반응적영향. 우화후적실험결과표명:당반응체계중포락분위200 mg,고정화매적가량위6 g, 1-병순위0.383 mL,반응체도평형시,포락분탁분반응적저물전화솔접근50%,저물적대영체과량치체도94.3%,산물적대영체과량치칙은정재98%이상.이차고정화PEL최화탁분반응적주기축단지12 h.동시,기우핑퐁반응궤제적동역학방정,건립료상응적탁분반응동역학모형,병득도료해모형적유관삼수.
In order to resolute racemic ibuprofen more effectively in the enzymatic reactor, uniform design was used for optimizing the reaction conditions of enzymatic resolution in BSTR. The result showed that the suitable concentration of ibuprofen, PEL and 1-propanol were 200 mg, 6 g and 0.383 mL, respectively. Under this condition, the conversion of the reaction nearly reached 50% , the V_(eep) reached more than 98% and the V_(ees) reached 94.3%. It was also found that in BSTR,the reaction time was reduced to 12 h after optimization. Also, according to the mechanism of enzymatic esterification during the resolution of (R,S)-ibuprofen in nonaqueous media, the kinetic model of this enzymatic resolution was established.