CAJ | 학술논문

认识和描述不同细菌芽胞α/β-SASP的分子结构特征,为深入开展以α/β-SASP为靶向修饰的应用技术提供科学依据.运用生物信息学方法和技术,比对分析4种菌株,炭疽芽胞杆菌Ames 株、苏云金芽胞杆菌serovar konkukian 97-27 株、腊样芽胞杆菌ATCC 10987株、枯草芽胞杆菌168 株的α/β-SASP基因及蛋白质一、二、三级结构的异同.基因-ClustalW2;一级结构-ClustalW2和ProtParam tool;二级结构-SOPMA;三级结构-SWISS-MODEL和Swiss-Pdbviewer4.0.1.4种菌株的α/β-SASP基因及蛋白质一、二、三级结构有明显的同源性,炭疽芽胞、苏云金芽胞和腊样芽胞的生物学特征非常相似.在开展细菌芽胞的α/β-SASP基因及生物效应研究时,可以首选苏云金杆菌芽胞或腊样杆菌芽胞作为炭疽杆菌芽胞的试验菌,其次可以选择枯草杆菌芽胞.
인식화묘술불동세균아포α/β-SASP적분자결구특정,위심입개전이α/β-SASP위파향수식적응용기술제공과학의거.운용생물신식학방법화기술,비대분석4충균주,탄저아포간균Ames 주、소운금아포간균serovar konkukian 97-27 주、석양아포간균ATCC 10987주、고초아포간균168 주적α/β-SASP기인급단백질일、이、삼급결구적이동.기인-ClustalW2;일급결구-ClustalW2화ProtParam tool;이급결구-SOPMA;삼급결구-SWISS-MODEL화Swiss-Pdbviewer4.0.1.4충균주적α/β-SASP기인급단백질일、이、삼급결구유명현적동원성,탄저아포、소운금아포화석양아포적생물학특정비상상사.재개전세균아포적α/β-SASP기인급생물효응연구시,가이수선소운금간균아포혹석양간균아포작위탄저간균아포적시험균,기차가이선택고초간균아포.
In order to provide scientific evidence for further developing techniques targeting at α/β-type small, acid-soluble spore proteins (SASPs) by understanding and describing the molecular structural characteristics of α/β-SASPs from four different Bacillus strains' spores. Bioinformatics methods and techniques were used to make a comparative analysis of genes, primary structures, secondary structures and tertiary structures of α/β-SASPs from four strains of Bacillus anthracis strains Ames, B. thuringiensis serovar konkukian strain 97-27, B. cereus ATCC 10987, and B. subtilis 168. Tools and softwares employed were: Clustal W2 for genes; Clustal W2 and ProtParam tool for primary structures; SOPMA for secondary structures; SWISS-MODEL and Swiss-Pdbviewer 4.0.1 for tertiary structures. The results showed that genes, primary, secondary, and tertiary structures of α/β-SASPs from four Bacillus strains had significant homology, and the biological characteristics of Bacillus anthracis, Bacillus thuringiensis and Bacillus cereus were very much resembled. Therefore, when doing researches on α/β-SASP gene of bacterial spore and its biological effects, one could first choose Bacillus anthracis, Bacillus thuriengsis, and Bacillus cereus, and secondly Bacillus subtilis.