CAJ | 학술논문

目的初步建立一种结核分支杆菌快速检测方法 .方法取两支无菌培养管,一支加入结核分支杆菌标准株(H37Rv)或者处理后的痰标本液;另一支加入蒸馏水作对照.两支试管均加入噬菌体D29,37℃培养90min,用硫酸亚铁铵杀死结核分支杆菌菌体外的噬菌体,柠檬酸三钠和氯化钙混和液中和硫酸亚铁铵.加入耻垢分支杆菌和硝酸钾,37℃培养6h,加入显色剂显色.同时应用噬菌体显色法和改良罗氏培养法检测20份临床标本,观察两种方法的检测效果.结果该方法灵敏度和特异性都为100%;临床标本的本法和罗氏检测表明两方法结果具有一致性(P＞0.01).结论噬菌体变色法可作为结核分支杆菌的快速检测方法 .
목적 초보건립일충결핵분지간균쾌속검측방법 .방법 취량지무균배양관,일지가입결핵분지간균표준주(H37Rv)혹자처리후적담표본액;령일지가입증류수작대조.량지시관균가입서균체D29,37℃배양90min,용류산아철안살사결핵분지간균균체외적서균체,저몽산삼납화록화개혼화액중화류산아철안.가입치구분지간균화초산갑,37℃배양6h,가입현색제현색.동시응용서균체현색법화개량라씨배양법검측20빈림상표본,관찰량충방법 적검측효과.결과 해방법령민도화특이성도위100%;림상표본적본법화라씨검측표명량방법결과구유일치성(P＞0.01).결론 서균체변색법가작위결핵분지간균적쾌속검측방법 .
Objective To establish a rapid detection of Mycobacterium tuberculosis .Methods add a standard strain of Mycobacterium tuberculosis(H37Rv)or post-treatment sputum fluid in tube,another add distilled water as control.Both test-tube was added 2 ul D29,37 t culture,to kill phage outside Mycobacterium tuberculosis bacterial with ferrous ammonium sulfate,Reacted ferrous ammonium sulfate with three citric acid sodium and calcium chloride.Added Mycobacterium smegmatis and potassium nitrate,37 ℃ 6h,added the color reagent.And 20 clinical specimens were detected to fined Mycobacterium tuberculosis with phage discoloration and L-J Mycobacteria culture method.Results The test results show that both the sensitivity and specificity were 100%;,and that the two methods is the consistency(P＞0.01).Conclusion Phage discoloration method for Mycobacterium tuberculosis may be rapid detection method.