CAJ | 학술논문

目的研究树状大分子修饰纳米金颗粒作为CT分子探针的可行性.方法将第5代聚酰胺胺树状大分子修饰的纳米金颗粒(Au DENPs)配制成15种浓度(0.001～0.1 mol/L)的悬液为实验组,相同浓度的非离子型碘造影剂(Omnipaque)为对照组,离体CT扫描比较相同浓度Au DENPs与Omnipaque的CT值差异.根据离体实验结果将6种浓度(0.006～0.02 mol/L)的Au DENPs 10 μl注射至BALB/C小鼠背部皮下行microCT扫描,观察其显影效果.结果 (1)离体实验发现当浓度≤0.01mol/L时,Au DENPs的CT值略低于相同浓度的非离子碘造影剂;当浓度>O.02mol/L时,Au DENPs的CT值高于相同浓度的非离子碘造影剂.(2)动物体内显像实验证实,当浓度≥0.009 mol/L时,经mieroCT成像可以清晰的显示注入小鼠皮下软组织的Au DENPs,而浓度≤0.008 mol/L时,注入小鼠体内的Au DENPs未被检出.结论 Au DENPs具有比现有CT造影剂更优秀的固有显影特性,具备成为CT分子探针的首要条件.
목적 연구수상대분자수식납미금과립작위CT분자탐침적가행성.방법 장제5대취선알알수상대분자수식적납미금과립(Au DENPs)배제성15충농도(0.001～0.1 mol/L)적현액위실험조,상동농도적비리자형전조영제(Omnipaque)위대조조,리체CT소묘비교상동농도Au DENPs여Omnipaque적CT치차이.근거리체실험결과장6충농도(0.006～0.02 mol/L)적Au DENPs 10 μl주사지BALB/C소서배부피하행microCT소묘,관찰기현영효과.결과 (1)리체실험발현당농도≤0.01mol/L시,Au DENPs적CT치략저우상동농도적비리자전조영제;당농도>O.02mol/L시,Au DENPs적CT치고우상동농도적비리자전조영제.(2)동물체내현상실험증실,당농도≥0.009 mol/L시,경mieroCT성상가이청석적현시주입소서피하연조직적Au DENPs,이농도≤0.008 mol/L시,주입소서체내적Au DENPs미피검출.결론 Au DENPs구유비현유CT조영제경우수적고유현영특성,구비성위CT분자탐침적수요조건.
Objective To study the feasibility of dendrimer-entrapped gold nanoparticles (Au DENPs) as a CT molecular probe. Methods Fifteen concentrations (0.001 to 0.1 mol/L) of gold nanoparticles were entrapped by amine-terminated polyamidoamine dendrimers of generation 5 in experiment groups. Same concentrations of non-ionic iodine contrast agent (Omnipaque) were set as control groups. The CT value of each concentrations of contrast agents in two groups were detected and compared in vitro. Six concentrations (0.006 ～ 0.02 mol/L) of Au DENPs at a dose of 10 μl were injected into the subcutaneous soft tissue of BALB/C mice back according to the experiment in vitro, then the experiment mice were scanned by a micro CT system to evaluate the development effect. Results (1) The CT values of Au DENPs were lower than those of non-ionic iodine contrast agent, when the concentration was 0.01 mol/L or lower in vitro. However, when the concentration exceeded 0.02 mol/L, the CT values of Au DENPs were higher than those of non-ionic iodine contrast agent. (2) Au DENPs at a dose of 10 μl injected into the subcutaneous soft tissue of mice could be detected by microCT, when the concentration of these particles was not less than 0.009 mol/L in vivo. At concentrations less than 0.008 mol/L, Au DENPs were undetectable in vivo. Conclusions Au DENPs show better inherent character for imaging than existing CT contrast agents, which preliminarily qualifies its use as a CT molecular probe.