中华创伤杂志
中華創傷雜誌
중화창상잡지
Chinese Journal of Traumatology
2010年
1期
70-75
,共6页
寇德伟%于腾波%李田米%于振萍
寇德偉%于騰波%李田米%于振萍
구덕위%우등파%리전미%우진평
脊髓损伤%转化生长因子β1%细胞移植%小神经胶质细胞
脊髓損傷%轉化生長因子β1%細胞移植%小神經膠質細胞
척수손상%전화생장인자β1%세포이식%소신경효질세포
Spinal curd injuries%Transforming growth factor betal%Cell transplantation%Microglia
目的 探讨活化小胶质细胞移植后脊髓组织内TGF-β1mRNA和蛋白表达及意义.方法 应用改良Allen法制造Wistar大鼠脊髓损伤模型,将活化小胶质细胞移植到脊髓损伤模型,测定脊髓组织内TGF-β1mRNA和蛋白表达.结果 各时相点对照组TGF-β1mRNA及其蛋白表达量明显低于移植组(P<0.01).结论 活化小胶质细胞移植后,可以上调损伤脊髓表达TGF-β1;脊髓内TGF-β1蛋白的高表达可能对脊髓损伤修复起促进作用;活化的小胶质细胞可能是TGF-β1的主要来源.
目的 探討活化小膠質細胞移植後脊髓組織內TGF-β1mRNA和蛋白錶達及意義.方法 應用改良Allen法製造Wistar大鼠脊髓損傷模型,將活化小膠質細胞移植到脊髓損傷模型,測定脊髓組織內TGF-β1mRNA和蛋白錶達.結果 各時相點對照組TGF-β1mRNA及其蛋白錶達量明顯低于移植組(P<0.01).結論 活化小膠質細胞移植後,可以上調損傷脊髓錶達TGF-β1;脊髓內TGF-β1蛋白的高錶達可能對脊髓損傷脩複起促進作用;活化的小膠質細胞可能是TGF-β1的主要來源.
목적 탐토활화소효질세포이식후척수조직내TGF-β1mRNA화단백표체급의의.방법 응용개량Allen법제조Wistar대서척수손상모형,장활화소효질세포이식도척수손상모형,측정척수조직내TGF-β1mRNA화단백표체.결과 각시상점대조조TGF-β1mRNA급기단백표체량명현저우이식조(P<0.01).결론 활화소효질세포이식후,가이상조손상척수표체TGF-β1;척수내TGF-β1단백적고표체가능대척수손상수복기촉진작용;활화적소효질세포가능시TGF-β1적주요래원.
Objective To investigate mRNA and protein expressions of TGF-β1 and their signifi-cance in injured spinal cord after transplantation of activated microglia. Methods Wistar rat model of spinal cord injury was made by using modified Allen's method. Then, the activated microglia was trans-planted into spinal cord injury models tu detect mRNA and protein expressions of TGF-β1 in the spinal curd. Results The mRNA and protein expressions of TGF-β1 in control group were significantly lower than that in test group (P<0.05). Conclusion Transplantation of activated microglia to the impaired spinal cord in rats can increase the mRNA and protein expressions of TGF-β1. The up-regulated expres-sion of TGF-β1 in spinal cord may play a role in promoting the recovery of spinal cord, when the activated microglia may be the main saurce of TGF-β1.
