中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2011年
2期
201-204
,共4页
佘高明%谢红%张力元%王琛
佘高明%謝紅%張力元%王琛
사고명%사홍%장력원%왕침
性别因素%认知障碍%七氟醚
性彆因素%認知障礙%七氟醚
성별인소%인지장애%칠불미
Sex factors%Cognition disorders%Sevoflurane
目的 评价吸入七氟醚对不同性别大鼠长期认知功能的影响.方法 健康成年SD大鼠42只,雌性大鼠22只(体重180～220 g),采用随机数字表法,将其随机分为2组(n=11):雌性对照组(Fc组)和雌性麻醉组(Fs组),雄性大鼠20只(体重380～440 g),采用随机数字表法,将其随机分为2组(n=10):雄性对照组(Mc组)和雄性麻醉组(Ms组).Fs组和Ms组吸入3%七氟醚2 h,Fc组和Mc组吸入95%氧气2 h,氧流量4 L/min.分别于停止给药后1、30、60、90 d时进行自发活动实验;分别于停止给药后2、30、60、90 d时进行避暗实验;分别于停止给药后3～7 d、31～35 d、61～65 d和91～95d时进行Morris水迷宫实验.结果 与Fc组比较,Fs组停止给药后1 d时活动总路程缩短,平均速度减慢,停止给药后3、31 d时逃避潜伏期和游泳总路程延长(P<0.05).与Mc组比较,Ms组停止给药后1 d时活动总路程缩短,平均速度减慢,停止给药后3～6 d、31～34 d、61 d时逃避潜伏期和游泳总路程延长(P<0.05).与Fs组比较,Ms组停止给药后4～6 d、34 d时逃避潜伏期和,游泳总路程延长(P<0.05).结论 吸入七氟醚可导致大鼠长期认知功能障碍,且对雄性大鼠的影响更明显.
目的 評價吸入七氟醚對不同性彆大鼠長期認知功能的影響.方法 健康成年SD大鼠42隻,雌性大鼠22隻(體重180～220 g),採用隨機數字錶法,將其隨機分為2組(n=11):雌性對照組(Fc組)和雌性痳醉組(Fs組),雄性大鼠20隻(體重380～440 g),採用隨機數字錶法,將其隨機分為2組(n=10):雄性對照組(Mc組)和雄性痳醉組(Ms組).Fs組和Ms組吸入3%七氟醚2 h,Fc組和Mc組吸入95%氧氣2 h,氧流量4 L/min.分彆于停止給藥後1、30、60、90 d時進行自髮活動實驗;分彆于停止給藥後2、30、60、90 d時進行避暗實驗;分彆于停止給藥後3～7 d、31～35 d、61～65 d和91～95d時進行Morris水迷宮實驗.結果 與Fc組比較,Fs組停止給藥後1 d時活動總路程縮短,平均速度減慢,停止給藥後3、31 d時逃避潛伏期和遊泳總路程延長(P<0.05).與Mc組比較,Ms組停止給藥後1 d時活動總路程縮短,平均速度減慢,停止給藥後3～6 d、31～34 d、61 d時逃避潛伏期和遊泳總路程延長(P<0.05).與Fs組比較,Ms組停止給藥後4～6 d、34 d時逃避潛伏期和,遊泳總路程延長(P<0.05).結論 吸入七氟醚可導緻大鼠長期認知功能障礙,且對雄性大鼠的影響更明顯.
목적 평개흡입칠불미대불동성별대서장기인지공능적영향.방법 건강성년SD대서42지,자성대서22지(체중180～220 g),채용수궤수자표법,장기수궤분위2조(n=11):자성대조조(Fc조)화자성마취조(Fs조),웅성대서20지(체중380～440 g),채용수궤수자표법,장기수궤분위2조(n=10):웅성대조조(Mc조)화웅성마취조(Ms조).Fs조화Ms조흡입3%칠불미2 h,Fc조화Mc조흡입95%양기2 h,양류량4 L/min.분별우정지급약후1、30、60、90 d시진행자발활동실험;분별우정지급약후2、30、60、90 d시진행피암실험;분별우정지급약후3～7 d、31～35 d、61～65 d화91～95d시진행Morris수미궁실험.결과 여Fc조비교,Fs조정지급약후1 d시활동총로정축단,평균속도감만,정지급약후3、31 d시도피잠복기화유영총로정연장(P<0.05).여Mc조비교,Ms조정지급약후1 d시활동총로정축단,평균속도감만,정지급약후3～6 d、31～34 d、61 d시도피잠복기화유영총로정연장(P<0.05).여Fs조비교,Ms조정지급약후4～6 d、34 d시도피잠복기화,유영총로정연장(P<0.05).결론 흡입칠불미가도치대서장기인지공능장애,차대웅성대서적영향경명현.
Objective To explore the difference in the effect of isoflurane inhalation on long-term cognitive function between male and female rate.Methods Forty-two SD rats (22 female, 20 male) that exhibited normal spontaneous activity and behaved normally in passive avoidance and Morris water maze tests were used in this study. They were divided into 2 sex groups:group female (group F) and group male (group M). Each group was further divided into 2 subgroups: control subgroup (Fc, Mc groups) and isoflurane group (Fs, Ms groups). The animals were anesthetized with 3 % isoflurane in O2 for 2 h in the 2 study subgroups, while the control subgroups inhaled O2 for 2 h. The spontaneous activity test was performed at 1, 30, 60 and 90 d, while the passive avoidance task was performed at 2, 30, 60 and 90 d after isoflurane anesthesia. Morris water maze test was performed for 5 consecutive days at 3-7 d, 31-35 d, 61-65 d, and 91-95 d after isoflurane anesthesia.Results In spontaneous activity test the total distance and the speed were significantly decreased at 1 d after isoflurane anesthesia in both Fs and Ms subgroups as compared with Fc and Mc subgroups. There was no significant difference in the number of error and latency after isoflurane anesthesia compared with the control subgroups in both male and female rats in the passive avoidance task. In Morris water maze test the escape latency and swimming distance were significantly prolonged at 1 and 31 d after isoflurane anesthesia as compared with control subgroup in female rats and at 3-6d, 31-34 d and 61 d after isoflurane anesthesia as compared with control subgroup in male rats, and were significantly longer after isoflurane anesthesia in male than in female rats. Conclusion Two hour 3.0% isoflurane anesthesia can impair long-term cognitive function and the impairment is greater in male than in female rats.
