中华儿科杂志
中華兒科雜誌
중화인과잡지
Chinese Journal of Pediatrics
2008年
7期
544-549
,共6页
王海燕%朱晓峰%王丽敏%罗志宏%杨自金%刘冬炎%原德新%聂磊%吴英杰%王淑贤
王海燕%硃曉峰%王麗敏%囉誌宏%楊自金%劉鼕炎%原德新%聶磊%吳英傑%王淑賢
왕해연%주효봉%왕려민%라지굉%양자금%류동염%원덕신%섭뢰%오영걸%왕숙현
缺氧缺血,脑%干细胞移植%侧脑室%脑源性神经营养因子%疾病模型,动物%大鼠
缺氧缺血,腦%榦細胞移植%側腦室%腦源性神經營養因子%疾病模型,動物%大鼠
결양결혈,뇌%간세포이식%측뇌실%뇌원성신경영양인자%질병모형,동물%대서
Hypoxia-ischemia,brain%Stem cell transplantation%Lateral ventricles%Brainderived neurotrophic factor%Di8ease models,animal:Rats
目的 观察脑源性神经营养因子(BDNF)及神经干细胞(NSCs)联合移植对缺氧缺血性脑损伤(HIBD)的治疗效果.方法 取新生24 h的Wistar大鼠海马NSCs进行培养、鉴定.选7日龄Wistar大鼠制备HIBD动物模型,模型制备后7 d行损伤侧侧脑室移植.将实验大鼠随机分为6组:正常组、模型组、假移植组、NSCs移植组、BDNF组、BDNF+NSCs移植组(联合移植组).移植后4周进行功能实验,取脑组织进行免疫组化免疫荧光检测.结果 从新生大鼠海马可成功培养出NSCs.Y迷宫实验结果:NSCs移植组达到学会的次数及正确记忆次数(163±11.60,5.00±1.13)n;BDNF组(150.00±8.94,5.17±1.47)n;BDNF+NSCs移植组(111.25±13.56,6.23±1.60)n.悬吊实验结果:NSCs移植组平均(30.10±11.8)s;BDNF组(36.25±10.98)s;BDNF+NSCs移植组(43.6±10.56)s.斜坡试验结果:NSCs移植组(20.3±8.25)s;BDNF组(14.33±2.88)s;BDNF+NSCs移植组(11.17±2.48)s.表明联合移植组大鼠学习记忆能力及神经功能与NSCs移植组比较明显改善(P＜0.05),而NSCs移植组大鼠学习记忆能力及神经功能与HIBD组比较,差异无统计学意义(P＞0.05).联合移植组损伤侧额叶皮层、海马存活的NSCs数量(9.31±0.71个,10.10±1.65个)与NSCs移植组(3.33±0.24个,4.22±0.33个)比较差异具有统计学意义(P＜0.01),且NSCs分化为神经元的比例明显增多,与NSCs移植组比较,差异有统计学意义(P＜0.05).结论 BDNF与NSCs联合移植较单独NSCs移植对HIBD有更好的疗效.
目的 觀察腦源性神經營養因子(BDNF)及神經榦細胞(NSCs)聯閤移植對缺氧缺血性腦損傷(HIBD)的治療效果.方法 取新生24 h的Wistar大鼠海馬NSCs進行培養、鑒定.選7日齡Wistar大鼠製備HIBD動物模型,模型製備後7 d行損傷側側腦室移植.將實驗大鼠隨機分為6組:正常組、模型組、假移植組、NSCs移植組、BDNF組、BDNF+NSCs移植組(聯閤移植組).移植後4週進行功能實驗,取腦組織進行免疫組化免疫熒光檢測.結果 從新生大鼠海馬可成功培養齣NSCs.Y迷宮實驗結果:NSCs移植組達到學會的次數及正確記憶次數(163±11.60,5.00±1.13)n;BDNF組(150.00±8.94,5.17±1.47)n;BDNF+NSCs移植組(111.25±13.56,6.23±1.60)n.懸弔實驗結果:NSCs移植組平均(30.10±11.8)s;BDNF組(36.25±10.98)s;BDNF+NSCs移植組(43.6±10.56)s.斜坡試驗結果:NSCs移植組(20.3±8.25)s;BDNF組(14.33±2.88)s;BDNF+NSCs移植組(11.17±2.48)s.錶明聯閤移植組大鼠學習記憶能力及神經功能與NSCs移植組比較明顯改善(P＜0.05),而NSCs移植組大鼠學習記憶能力及神經功能與HIBD組比較,差異無統計學意義(P＞0.05).聯閤移植組損傷側額葉皮層、海馬存活的NSCs數量(9.31±0.71箇,10.10±1.65箇)與NSCs移植組(3.33±0.24箇,4.22±0.33箇)比較差異具有統計學意義(P＜0.01),且NSCs分化為神經元的比例明顯增多,與NSCs移植組比較,差異有統計學意義(P＜0.05).結論 BDNF與NSCs聯閤移植較單獨NSCs移植對HIBD有更好的療效.
목적 관찰뇌원성신경영양인자(BDNF)급신경간세포(NSCs)연합이식대결양결혈성뇌손상(HIBD)적치료효과.방법 취신생24 h적Wistar대서해마NSCs진행배양、감정.선7일령Wistar대서제비HIBD동물모형,모형제비후7 d행손상측측뇌실이식.장실험대서수궤분위6조:정상조、모형조、가이식조、NSCs이식조、BDNF조、BDNF+NSCs이식조(연합이식조).이식후4주진행공능실험,취뇌조직진행면역조화면역형광검측.결과 종신생대서해마가성공배양출NSCs.Y미궁실험결과:NSCs이식조체도학회적차수급정학기억차수(163±11.60,5.00±1.13)n;BDNF조(150.00±8.94,5.17±1.47)n;BDNF+NSCs이식조(111.25±13.56,6.23±1.60)n.현조실험결과:NSCs이식조평균(30.10±11.8)s;BDNF조(36.25±10.98)s;BDNF+NSCs이식조(43.6±10.56)s.사파시험결과:NSCs이식조(20.3±8.25)s;BDNF조(14.33±2.88)s;BDNF+NSCs이식조(11.17±2.48)s.표명연합이식조대서학습기억능력급신경공능여NSCs이식조비교명현개선(P＜0.05),이NSCs이식조대서학습기억능력급신경공능여HIBD조비교,차이무통계학의의(P＞0.05).연합이식조손상측액협피층、해마존활적NSCs수량(9.31±0.71개,10.10±1.65개)여NSCs이식조(3.33±0.24개,4.22±0.33개)비교차이구유통계학의의(P＜0.01),차NSCs분화위신경원적비례명현증다,여NSCs이식조비교,차이유통계학의의(P＜0.05).결론 BDNF여NSCs연합이식교단독NSCs이식대HIBD유경호적료효.
Objective To investigate the effects of brain-derived neurotrophic factor(BDNF)on survival,migration and differentiation on of neural stem cells(NSCs)transplanted into the brain of newborn rats with hypoxic.ischeroic brain damage and the recovery of nervous functions.Methods The NSCs were separated from hippocampus of neonatal Wistar rats within 24 h after birtk Brdu.NSE and GFAP were used as markers of differentiation and proliferation of NSCs.The newborn rats were subjected to hypoxic-ischemic condition to induce brain damage.Seven days later,NSCs transplantation wag performed for the animals The rats wera divided into normal control group,HIBD group,PBS group,NSCs transplantation group,BDNF group and BDNF+NSCs transplantation group randomly.At4 weeks after Uznsplantation the nervous function of rats was observed bv Y-maze and nerve behavior test.After they were sacrificed.the rat brains were examined by immunocytochemistry for Brdu and by immunofluorescence for NSE/Brdu.Results The hippocampus NSCs of newborn rat could be well cultured and thev expressed nestin and they could differentiate into NSE.GFAP.Most of NSCs survived in cerebral ventricle 4 weeks after transplantation in brain through Brdu innnunocytochemistry and they migrated into regions of brain extensively,especially to the injured side of cortex and hippocampus.The number of living NSCs in the injured side of cortex and hippocampus of BDNF+NSCs transplantation group increased evidently and the percentage of NSCs differentiated into NSE was higher than that in the NSCs transplantation group(P＜0.05).The nerve function recovery of the rats in BDNF and NSCs treated group Wna significantly better than that in the other groups(P＜0.05).The NSCs group had no prominent changes as compared with the model groups(P＞0.05).Conclusions NSCs can beisolatedfrom newborn rats hippocampus and cultured in vivo.NSCs Can survive.migrate and differentiate into neurons through cerebral ventricle. BDNF could significantly accderate proliferation and differentiation of NSCs transplanted into the brain ofrats with HIBD.The nervous function recovery Was improved prominently by transplantation of NSCs with BDNF application,which may become a potentiatly effective method to treat HIBD.
