中国医药
中國醫藥
중국의약
CHINA MEDICINE
2012年
10期
1265-1267
,共3页
么文博%胡万宁%张庆波%苏铁柱%徐丽晓
麽文博%鬍萬寧%張慶波%囌鐵柱%徐麗曉
요문박%호만저%장경파%소철주%서려효
细胞毒性T淋巴细胞%肿瘤特异性抗原%超抗原%杀伤活性
細胞毒性T淋巴細胞%腫瘤特異性抗原%超抗原%殺傷活性
세포독성T림파세포%종류특이성항원%초항원%살상활성
Cytotoxic T lymphocyte cells%Tumor specific antigen%Super-antigen%Killing activity
目的 探讨肿瘤可溶性抗原(TSA)联合超抗原金黄色葡萄球菌肠毒素C(SEC)诱导的细胞毒性T淋巴细胞(CTLs)对肿瘤细胞的杀伤作用.方法 实验分为对照组(淋巴细胞)和实验组(SEC+ TSA+淋巴细胞).分离肿瘤患者外周血淋巴细胞,经TSA、超抗原SEC联合作用诱导产生CTLs,对其增殖、细胞表型、杀瘤活性进行观察和测定.结果 经TSA、SEC联合刺激的淋巴细胞组增殖活性明显增强.实验组和对照组CD3均阳性表达,实验组CD8+明显高于对照组(49.07％比27.52％,P＜0.05).经肺癌TSA、超抗原SEC联合刺激淋巴细胞组培养诱导的CTLs,当效靶比为20∶1时对CALU-6、自体肺癌细胞、Hela细胞杀伤活性明显高于效靶比为10∶1[分别(89.6±3.7)％比(51.5±4.0)％,(92.0±4.0)％比(54.5±4.0)％,(65.0±3.8)％比(35.3±2.4)％,均P＜0.05]；效应细胞对人肺癌细胞株CALU-6、自体肺癌细胞的杀伤活性明显高于Hela细胞(P＜0.05).结论 经肿瘤抗原和超抗原SEC诱导的CTL能够产生高效特异性的杀瘤效应.
目的 探討腫瘤可溶性抗原(TSA)聯閤超抗原金黃色葡萄毬菌腸毒素C(SEC)誘導的細胞毒性T淋巴細胞(CTLs)對腫瘤細胞的殺傷作用.方法 實驗分為對照組(淋巴細胞)和實驗組(SEC+ TSA+淋巴細胞).分離腫瘤患者外週血淋巴細胞,經TSA、超抗原SEC聯閤作用誘導產生CTLs,對其增殖、細胞錶型、殺瘤活性進行觀察和測定.結果 經TSA、SEC聯閤刺激的淋巴細胞組增殖活性明顯增彊.實驗組和對照組CD3均暘性錶達,實驗組CD8+明顯高于對照組(49.07％比27.52％,P＜0.05).經肺癌TSA、超抗原SEC聯閤刺激淋巴細胞組培養誘導的CTLs,噹效靶比為20∶1時對CALU-6、自體肺癌細胞、Hela細胞殺傷活性明顯高于效靶比為10∶1[分彆(89.6±3.7)％比(51.5±4.0)％,(92.0±4.0)％比(54.5±4.0)％,(65.0±3.8)％比(35.3±2.4)％,均P＜0.05]；效應細胞對人肺癌細胞株CALU-6、自體肺癌細胞的殺傷活性明顯高于Hela細胞(P＜0.05).結論 經腫瘤抗原和超抗原SEC誘導的CTL能夠產生高效特異性的殺瘤效應.
목적 탐토종류가용성항원(TSA)연합초항원금황색포도구균장독소C(SEC)유도적세포독성T림파세포(CTLs)대종류세포적살상작용.방법 실험분위대조조(림파세포)화실험조(SEC+ TSA+림파세포).분리종류환자외주혈림파세포,경TSA、초항원SEC연합작용유도산생CTLs,대기증식、세포표형、살류활성진행관찰화측정.결과 경TSA、SEC연합자격적림파세포조증식활성명현증강.실험조화대조조CD3균양성표체,실험조CD8+명현고우대조조(49.07％비27.52％,P＜0.05).경폐암TSA、초항원SEC연합자격림파세포조배양유도적CTLs,당효파비위20∶1시대CALU-6、자체폐암세포、Hela세포살상활성명현고우효파비위10∶1[분별(89.6±3.7)％비(51.5±4.0)％,(92.0±4.0)％비(54.5±4.0)％,(65.0±3.8)％비(35.3±2.4)％,균P＜0.05]；효응세포대인폐암세포주CALU-6、자체폐암세포적살상활성명현고우Hela세포(P＜0.05).결론 경종류항원화초항원SEC유도적CTL능구산생고효특이성적살류효응.
Objective To study the cytotoxic T lymphocyte cells(CTLs)induced by tumor soluble antigen (TSA)and super-antigen staphylococcal enterotoxin C(SEC)and its anti-tumor effect in vitro.Methods The experiment was divided into control group(lymphocyte cells)and experiment group(SEC + TSA + lymphocyte cells).Lymphocyte cells were isolated from peripheral blood of cancer patients and then were induced by TSA and SEC in vitro, the levels of proliferation, cell phenotype and killing activity of CTLs were tested. Results Proliferation activity of lymphocyte cells group stimulated by TSA and SEC was the strongest.CD3 was expressed in control group and experiment group,and the CDs+ expressed in experiment group was higher than that in control group(49.07％ vs 27.52％,P ＜0.05).When the ratio of effect and target was 20:1,the kill rates of CTLs on CALU-6,the lung cancer cells and Hela cells were higher than those when the ration was 10:1,and the kill rates of CTLs on CALU-6,the lung cancer cells were higher than that of Hela cells(P ＜ 0.05).Conclusion The CTLs modified by super-antigen SEC and tumor antigen can induce high anti-tumor efficiency.
