中国实用医刊
中國實用醫刊
중국실용의간
CENTRAL PLAINS MEDICAL JOURNAL
2011年
4期
21-23
,共3页
刘德义%付月云%彭燕%张海生%黄河
劉德義%付月雲%彭燕%張海生%黃河
류덕의%부월운%팽연%장해생%황하
肺肿瘤%腺癌%血清%双向凝胶电泳%基质辅助激光解吸电离飞行时间质谱仪%蛋白质组
肺腫瘤%腺癌%血清%雙嚮凝膠電泳%基質輔助激光解吸電離飛行時間質譜儀%蛋白質組
폐종류%선암%혈청%쌍향응효전영%기질보조격광해흡전리비행시간질보의%단백질조
Lung neoplasm%Adenocarcinoma%Serum%Two-dimensional gel electrophoresis%Matrix-assisted laser desorption/ionization time of flight mass spectrometry%Proteome
目的 分析肺腺癌患者与正常人血清双向凝胶电泳的差异蛋白质,从而寻找肺腺癌早期特异性的诊断指标.方法 用固相IPG梯度双向凝胶电泳分离肺腺癌患者及正常人血清总蛋白,用PDQuest凝胶图像软件分析,以识别差异蛋白质点.用基质辅助激光解吸电离飞行时间质谱仪(MALDI-TOF-MS)分析获得肽质量指纹图,然后利用Mascot查询软件搜寻数据库鉴定蛋白质.结果 ①获得了重复性较好的双向电泳银染图谱.②经PDQuest 7.1软件匹配分析,共筛选出22个差异的蛋白点,对7个差异蛋白点进行胶内原位酶解-质谱指纹图分析,获得了7张肽质量指纹图谱,查询数据库鉴定出1个蛋白质-血清淀粉样蛋白A(SAA).结论 ①固相pH梯度双向凝胶电泳分离血清总蛋白获得了重复性较好的双向电泳凝胶图谱.②肺腺癌患者血清和正常人血清的蛋白质组表达存在明显差异,这些差异蛋白点为进一步建立人肺癌的2-DE数据库奠定了基础.③SAA蛋白很可能是肺癌的一个潜在标志物.
目的 分析肺腺癌患者與正常人血清雙嚮凝膠電泳的差異蛋白質,從而尋找肺腺癌早期特異性的診斷指標.方法 用固相IPG梯度雙嚮凝膠電泳分離肺腺癌患者及正常人血清總蛋白,用PDQuest凝膠圖像軟件分析,以識彆差異蛋白質點.用基質輔助激光解吸電離飛行時間質譜儀(MALDI-TOF-MS)分析穫得肽質量指紋圖,然後利用Mascot查詢軟件搜尋數據庫鑒定蛋白質.結果 ①穫得瞭重複性較好的雙嚮電泳銀染圖譜.②經PDQuest 7.1軟件匹配分析,共篩選齣22箇差異的蛋白點,對7箇差異蛋白點進行膠內原位酶解-質譜指紋圖分析,穫得瞭7張肽質量指紋圖譜,查詢數據庫鑒定齣1箇蛋白質-血清澱粉樣蛋白A(SAA).結論 ①固相pH梯度雙嚮凝膠電泳分離血清總蛋白穫得瞭重複性較好的雙嚮電泳凝膠圖譜.②肺腺癌患者血清和正常人血清的蛋白質組錶達存在明顯差異,這些差異蛋白點為進一步建立人肺癌的2-DE數據庫奠定瞭基礎.③SAA蛋白很可能是肺癌的一箇潛在標誌物.
목적 분석폐선암환자여정상인혈청쌍향응효전영적차이단백질,종이심조폐선암조기특이성적진단지표.방법 용고상IPG제도쌍향응효전영분리폐선암환자급정상인혈청총단백,용PDQuest응효도상연건분석,이식별차이단백질점.용기질보조격광해흡전리비행시간질보의(MALDI-TOF-MS)분석획득태질량지문도,연후이용Mascot사순연건수심수거고감정단백질.결과 ①획득료중복성교호적쌍향전영은염도보.②경PDQuest 7.1연건필배분석,공사선출22개차이적단백점,대7개차이단백점진행효내원위매해-질보지문도분석,획득료7장태질량지문도보,사순수거고감정출1개단백질-혈청정분양단백A(SAA).결론 ①고상pH제도쌍향응효전영분리혈청총단백획득료중복성교호적쌍향전영응효도보.②폐선암환자혈청화정상인혈청적단백질조표체존재명현차이,저사차이단백점위진일보건립인폐암적2-DE수거고전정료기출.③SAA단백흔가능시폐암적일개잠재표지물.
Objective To analyze the proteomic components of the serum from the patients with lung adenocarcinoma and healthy donors, in search of the specific tumor markers of lung adenocarcinoma for diagnose. Methods The serum total protein of human lung adenocarcinoma serum and healthy donors serum were separated by immobilized pH gradient based on two-dimensional polyacrylamide gel electrophoresis(2-DE).The differentially expressed proteins were analyzed using PDQuest analysis software, then identified using MALDI-TOF-MS and Mascot differentially expressed in serum from lung adenocarcinoma patients and healthy donors. Seven protein spots of interest were incised from gel and digested in-gel by trypsin, seven PMF maps were obtained by MALDI-TOF-MS.The typical peptide masses were searched in the NCBInr database by Mascot software,one protein was identified succesfully.It was serum amyloid A(SAA). Conclusions two-dimensional polyacrylamide gel electrophoresis to separate and identify the between lung adenocarcinomma patients and healthy donors.It will be useful for biomark of lung cancer.