CAJ | 학술논문

目的：观察乙型肝炎病毒(HBV)S区DNA疫苗pCR3.1-S诱导BALB/C小鼠(H-2d)的特异性免疫应答，及其对稳定表达HBsAg的小鼠肥大细胞瘤细胞P815(P815-HBV-S)成瘤性的影响。方法：肌肉注射DNA疫苗；背部皮下接种P815-HBV-S细胞，观察成瘤情况；ELISA法检测小鼠血清抗HBs;4h51Cr释放法检测小鼠脾细胞CTL活性。结果：pCR3.1-S体外可表达HBsAg；小鼠接种该疫苗后血清450nmrA值为0.38，强化免疫后达0.87；pCR3.1-S组CTL细胞杀伤活性为51.1％，对照组为20.5％(P＜0.01)。接种P815-HBV-S细胞后对照组成瘤率1100％,pCR3.1-S小鼠成瘤率为16.70%6，对照组小鼠6周后全部死亡；pCR3.1-S组6周后存活率为87.5％。结论：HBV S区DNA疫苗具有良好的免疫原性，能够诱导小鼠产生特异性体液免疫和细胞免疫应答，对体内HBV感染具有预防及治疗作用。
목적：관찰을형간염병독(HBV)S구DNA역묘pCR3.1-S유도BALB/C소서(H-2d)적특이성면역응답，급기대은정표체HBsAg적소서비대세포류세포P815(P815-HBV-S)성류성적영향。방법：기육주사DNA역묘；배부피하접충P815-HBV-S세포，관찰성류정황；ELISA법검측소서혈청항HBs;4h51Cr석방법검측소서비세포CTL활성。결과：pCR3.1-S체외가표체HBsAg；소서접충해역묘후혈청450nmrA치위0.38，강화면역후체0.87；pCR3.1-S조CTL세포살상활성위51.1％，대조조위20.5％(P＜0.01)。접충P815-HBV-S세포후대조조성류솔1100％,pCR3.1-S소서성류솔위16.70%6，대조조소서6주후전부사망；pCR3.1-S조6주후존활솔위87.5％。결론：HBV S구DNA역묘구유량호적면역원성，능구유도소서산생특이성체액면역화세포면역응답，대체내HBV감염구유예방급치료작용。
Objective:To observe the specific immune responses and the protection against P815 mastocytoma cells stable expressing HbsAg in H-2d mice after immunized by DNA vaccine of HBV S gene. Methods: The immunization was performed by intramuscular injection of DNA and subcutaneous injection of P815-HBV-S. Serum anti-HBs was detected by ELISA. HbsAg secific cytotoxic T lymphocyte(CTL) activity was measured by 51 Chromium release assay. Results:The expression of HBsAg was demonstrated by indirect immunofluorescent method. The 450nm A value of serum in immunized mice was 0.87.HBsAg specific CTL activity was 51.1%. HBV-SDNA vaccine could evidently inhibit the tumor growth, prolong the surviv al period(＞38.2 d), and improve the survival rate. Conclusion: pCR3.1-S DNA vaccine has a strong antigenicity and marked killing effect to HBV infected cells in vivo.