CAJ | 학술논문

用免疫组织化学S-P法,应用七种特异性胃肠激素抗血清对武夷湍蛙胃肠道7个部位内分泌细胞的局部分布、分布密度及形态学特征进行了研究.结果显示:在武夷湍蛙胃肠道中鉴别出四种内分泌细胞,即:5-羟色胺、生长抑素、胃泌素和高血糖素免疫反应细胞.P-物质、胰岛素和胰多肽免疫反应细胞未见.5-羟色胺免疫反应细胞是胃肠道中最主要的内分泌细胞类型,其在胃肠道各段均有分布,但在各段分布密度不同.生长抑制素免疫反应细胞分布于贲门至十二指肠的胃肠道各段.胃泌素免疫反应细胞局限分布于幽门和十二指肠部位.高血糖素免疫反应细胞仅在胃幽门部位有分布.在武夷湍蛙胃肠道内具有最多种类型内分泌细胞分布的部位是幽门,同时胃肠道内各种内分泌细胞在此部位也显示出最高的分布密度.武夷湍蛙胃肠道内分泌细胞形态多样:圆形、椭圆形、纺锤形、梭形、锥形和不规则形.胃部多数内分泌细胞分布于胃腺中,肠道中多数细胞则分布于上皮细胞间,少数分布于固有膜.本研究显示武夷湍蛙与其他两栖动物胃肠道内分泌细胞在分布模式上存在一定共同特征并具其独特性,以上结果提示其与武夷湍蛙消化生理学相关,和其胃肠道的调节特点相关.
용면역조직화학S-P법,응용칠충특이성위장격소항혈청대무이단와위장도7개부위내분비세포적국부분포、분포밀도급형태학특정진행료연구.결과현시:재무이단와위장도중감별출사충내분비세포,즉:5-간색알、생장억소、위비소화고혈당소면역반응세포.P-물질、이도소화이다태면역반응세포미견.5-간색알면역반응세포시위장도중최주요적내분비세포류형,기재위장도각단균유분포,단재각단분포밀도불동.생장억제소면역반응세포분포우분문지십이지장적위장도각단.위비소면역반응세포국한분포우유문화십이지장부위.고혈당소면역반응세포부재위유문부위유분포.재무이단와위장도내구유최다충류형내분비세포분포적부위시유문,동시위장도내각충내분비세포재차부위야현시출최고적분포밀도.무이단와위장도내분비세포형태다양:원형、타원형、방추형、사형、추형화불규칙형.위부다수내분비세포분포우위선중,장도중다수세포칙분포우상피세포간,소수분포우고유막.본연구현시무이단와여기타량서동물위장도내분비세포재분포모식상존재일정공동특정병구기독특성,이상결과제시기여무이단와소화생이학상관,화기위장도적조절특점상관.
Using 7 types of antisera, the regional distribution and distributional density as well as morphological features of endocrine cells in 7 parts of the gastrointestinal track (GIT) of Mt. Wuyi cascade-frog (Amolops wuyiensis) were investigated by streptavidin- peroxidase (S-P) immunohistochemical method. The result showed that four types of gastrointestinal (GI) endocrine cells were identified in the GIT of A. wuyiensis, e.g. 5-hydroxytryptamine (5-HT), somatostatin(SS), gastrin(GAS), glucagon(GLU) and immunoreactive(IR) cells. Substance P (SP)-, Pancreatic Polypeptide (PP)- and Insulin (INS)-IR cells were not detected. 5-HT-IR cells distributed throughout the whole GIT at various densities and they were the most predominant cell types in the GIT. SS-IR cells were detected from cardia to duodenum. GAS-IR cells showed a restricted distribution and were only demonstrated in the pylorus and duodenum. GLU-IR cells were only confined to pylorus. In the GIT of A. wuyiensis, the region with the highest degree of cell type heterogeneity was pylorus and all types of endocrine cell along the GIT showed peak density in pylorus as well. The endocrine cells were round or oval, spindle, shuttle, pyramid or irregularity in shape. And they were mostly found in the gastric glands of the stomach regions and in the epithelium of intestine, and occasionally occurred in lamina propria. The present study showed that some common features on the distributional pattern of the different types of GI endocrine cells were found between A. wuyiensis. and other amphibians, and on the other hand, some species-dependent unique distributional patterns of endocrine cells were also detected in GIT of A. wuyiensis. The results suggested that these characteristics might be related to the GIT physiology and the regulatory characteristics of the GIT of the A. wuyiensis.