CAJ | 학술논문

在生理酸度条件下(pH 7.4), 采用溴化乙锭(EB)为荧光探针的荧光光谱法、 I-离子荧光猝灭效应、 DNA 熔点和粘度效应等手段, 研究了三聚氰胺与DNA的相互作用. 随着DNA的加入, 三聚氰胺的荧光强度明显减小而且三聚氰胺能够猝灭DNA-EB复合物的荧光, 说明三聚氰胺能够竞争置换EB而与DNA作用;三聚氰胺的加入使得DNA的粘度增大, DNA-EB的熔点降低;DNA的加入减小了I~-对三聚氰胺荧光的猝灭程度. 三聚氰胺以嵌插方式作用于DNA的亲核位点, 意味着三聚氰胺进入生物体后有可能通过形成DNA加合物的形式造成DNA损伤, 从而最终导致基因突变.
재생리산도조건하(pH 7.4), 채용추화을정(EB)위형광탐침적형광광보법、 I-리자형광졸멸효응、 DNA 용점화점도효응등수단, 연구료삼취청알여DNA적상호작용. 수착DNA적가입, 삼취청알적형광강도명현감소이차삼취청알능구졸멸DNA-EB복합물적형광, 설명삼취청알능구경쟁치환EB이여DNA작용;삼취청알적가입사득DNA적점도증대, DNA-EB적용점강저;DNA적가입감소료I~-대삼취청알형광적졸멸정도. 삼취청알이감삽방식작용우DNA적친핵위점, 의미착삼취청알진입생물체후유가능통과형성DNA가합물적형식조성DNA손상, 종이최종도치기인돌변.
The interaction between melamine and calf thymus DNA in physiological buffer (pH 7.4) was explored by using ethidium bromide (EB) as a fluorescence probe. The experimental phenomena showed that fluorescence intensity of melamine was greatly reduced with the addition of DNA. Moreover, fluorescence quenching of the emission peak was observed in the DNA-EB system when melamine was added, suggesting that a strong competition for DNA binding between melamine and EB. The relative viscosity of DNA increased with the addition of melamine, whereas the value of melting temperature of DNA-EB system decreased in the presence of melamine. In addition, I~- quenching effect on the fluorescence intensity of melamine was reduced in the presence of DNA. Based on the above experimental phenomena, it can be inferred that the binding mode of melamine with DNA is intercalative binding, the melamine can bind to base pairs of DNA to produce DNA adducts. The melamine might bring the chemical trauma to DNA of the organisms and induce the gene mutation through producing DNA adducts.