CAJ | 학술논문

采用PHA体内直接注射法制备了七带石斑鱼(Epinephelus septemfasciatus)头肾组织染色体标本并分析其核型.结果表明,七带石斑鱼二倍体染色体数为48,24对染色体均为端部着丝粒染色体,其染色体臂数(NF)为48,核型公式为:2n=48t,NF=48.未发现与性别相关的异型染色体,第24对染色体长度明显小于其他染色体.通过与其他22种石斑鱼染色体核型进行比较,发现七带石斑鱼具有石斑鱼属鱼类的原始核型特征,属于石斑鱼属鱼类的原始类群.
채용PHA체내직접주사법제비료칠대석반어(Epinephelus septemfasciatus)두신조직염색체표본병분석기핵형.결과표명,칠대석반어이배체염색체수위48,24대염색체균위단부착사립염색체,기염색체비수(NF)위48,핵형공식위:2n=48t,NF=48.미발현여성별상관적이형염색체,제24대염색체장도명현소우기타염색체.통과여기타22충석반어염색체핵형진행비교,발현칠대석반어구유석반어속어류적원시핵형특정,속우석반어속어류적원시류군.
The metaphase chromosomes of Epinephelus septemfasciatus were obtained from head kidney tissue by the method of PHA and colchicine injection and the karyotype was analyzed.The results showed that there were 48 telocentric chromosomes in the diploid and their karyotypic formula was 2n=48t,NF=48.The length of the 24th chromosome was distinctively shorter than that of others,while there was no obvious difference in length among the other 23 chromosomes.By comparison with the chromosome karyotypes of other 22 Epinephelus species,it can be concluded E.septemfasciatus has the representative characteristics of ancestral karyotypic pattern and belongs to the primitive grouper.