CAJ | 학술논문

[目的]克隆拟南芥CBF3基因并构建植物表达载体pCAMBIA1301-Rd29A-CBF3.[方法]从模式植物拟南芥中克隆分离出转录因子CBF3与逆境诱导型启动子Rd29A的DNA序列,构建植物表达载体.[结果]克隆得到的CBF3与GenBank数据库所公布序列比对发现,同源性达到100%,全长为750bp;克隆克隆得到的Rd29A与GenBank数据库公布序列比对发现,同源性达到100%,全长为1425 bp.[结论]以双元载体pCAMBIA1301为基础,植物表达载体pCAMBIA1301-Rd29A-CBF3被成功构建,对提高植物耐盐、耐旱和耐寒性有很大帮助.
[목적]극륭의남개CBF3기인병구건식물표체재체pCAMBIA1301-Rd29A-CBF3.[방법]종모식식물의남개중극륭분리출전록인자CBF3여역경유도형계동자Rd29A적DNA서렬,구건식물표체재체.[결과]극륭득도적CBF3여GenBank수거고소공포서렬비대발현,동원성체도100%,전장위750bp;극륭극륭득도적Rd29A여GenBank수거고공포서렬비대발현,동원성체도100%,전장위1425 bp.[결론]이쌍원재체pCAMBIA1301위기출,식물표체재체pCAMBIA1301-Rd29A-CBF3피성공구건,대제고식물내염、내한화내한성유흔대방조.
[Objective] The aim was to clone CBF3 gene from Arabidopsis thaliana and construct plant expression vector pCAMBIA1301-Rd29ACBF3. [Method] CBF3 gene and stress-inducible promoter Rd29A were amplified from the genomic DNA of A. thaliana for the construction of plant expression vector. [Result] Sequencing results showed that the cloned CBF3 gene had 750 bp, and showed 100% identity with the sequence published on GenBank. The promoter Rd29A had 1 425 bp, and showed 100% identity with the sequence published on GenBank. [Conclusion] Based on the binary vector pCAMBIA1301, the plant expression vector pCAMBIA1301-Rd29A-CBF3 was constructed successfully,which could materially improve the salt resistance, drought-tolerance, cold resistance of plants.