CAJ | 학술논문

应用PCR从赤红球菌JDM312株基因组DNA中扩增出chnB基因序列,构建pUC18-chnB重组质粒,转化大肠杆菌DH5α,并对插入片段进行测序,用Clustal X和Mega 3.1软件对测定DNA序列进行相似性和系统发育分析.结果显示：扩增得到的chnB基因大小为1 623 bp,编码由542个氨基酸残基构成的多肽,与已报道不同细菌属菌种chnB基因核苷酸序列的相似性为85%～98%,其中与红球菌Phi2株的亲缘关系最近.
응용PCR종적홍구균JDM312주기인조DNA중확증출chnB기인서렬,구건pUC18-chnB중조질립,전화대장간균DH5α,병대삽입편단진행측서,용Clustal X화Mega 3.1연건대측정DNA서렬진행상사성화계통발육분석.결과현시：확증득도적chnB기인대소위1 623 bp,편마유542개안기산잔기구성적다태,여이보도불동세균속균충chnB기인핵감산서렬적상사성위85%～98%,기중여홍구균Phi2주적친연관계최근.
The chnB gene encoding a cyclohexanone monooxygenase was amplified by PCR from genomic DNA of Rhodococcus ruber JDM312,the PCR product was cloned into the pUC18 vector and sequenced,and the sequence identity searches were performed by using software Clustal X and Mega 3.1.The sequence analysis showed that the open reading frame of the chnB gene from Rhodococcus ruber JDM312 was 1 623 bp and codes for a polypeptide of 542 amino acids.DNA identities of the chnB genes between the JDM312 strain and the previously reported four other stains of Rhodococcus was 85% to 98%,and the phylogenic tree showed that these strains clustered one main branch.It can be concluded that the chnB gene of Rhodococcus ruber JDM312 is useful to further study on expression of cyclohexanone monooxygenase and its enzymatic activity.