CAJ | 학술논문

目的探讨β2-AR 27位点基因多态性与哮喘的关系及其对气道高反应性的保护作用.方法采用多聚酶链反应(PCR)-等位基因扩增法,对149例具有气道高反应性的咳嗽变异型哮喘患者进行β2-AR 27位点基因多态性分析检测,同时追踪2年,观察其发展成典型哮喘的比率,并与90名健康对照者进行比较.结果 (1)中国南方汉族人群β2-AR 27位点基因多态性分布以杂合子所占比例较高(57%),突变型纯合子比率较小(20%);(2)哮喘组β2-AR 27位点谷氨酸/谷氨酸基因型频率,明显低于健康对照组(9%VS20%),OR为0.4(P＜0.05),95%CI为(0.2～0.9),谷氨酸等位基因频率与对照组差异无统计学意义(P＞0.05);(3)β2-AR 27位点基因多态性在典型哮喘组谷氨酸/谷氨酸基因型分布频率明显低于哮喘稳定组(P＜0.05).结论中国南方汉族人群中27位点基因多态性与哮喘关联,Glu27可能具有气道保护作用.
목적 탐토β2-AR 27위점기인다태성여효천적관계급기대기도고반응성적보호작용.방법 채용다취매련반응(PCR)-등위기인확증법,대149례구유기도고반응성적해수변이형효천환자진행β2-AR 27위점기인다태성분석검측,동시추종2년,관찰기발전성전형효천적비솔,병여90명건강대조자진행비교.결과 (1)중국남방한족인군β2-AR 27위점기인다태성분포이잡합자소점비례교고(57%),돌변형순합자비솔교소(20%);(2)효천조β2-AR 27위점곡안산/곡안산기인형빈솔,명현저우건강대조조(9%VS20%),OR위0.4(P＜0.05),95%CI위(0.2～0.9),곡안산등위기인빈솔여대조조차이무통계학의의(P＞0.05);(3)β2-AR 27위점기인다태성재전형효천조곡안산/곡안산기인형분포빈솔명현저우효천은정조(P＜0.05).결론 중국남방한족인군중27위점기인다태성여효천관련,Glu27가능구유기도보호작용.
Objective To analyze the association between β2-AK 27 locus genetic polymorphisms and asthma, and the protective effect in airway hyperreactivity. Methods The allel polymerase chain reaction were used to determine β2-AR 27 locus genetic polymorphisms in 149 patients with cough variant asthma who have the airway hyperreactivity. To observe these people for two years in order to know the proportion of changed to typical asthma. And compare with 90 people in healthy group. Results (1) The distribution frequency of β2-AR 27 locus genetic polymorphisms is major in heterozygote (57 % ) , and the Glu/Glu homozygote has the least ( 20% ) , (2) There was a significant decrease in the frequency of Glu/Glu genotype in asthmatics compared with healthy group(9% VS 20% ) ,OR = 0.4(P<0.05) ,95% CI (0.2 ～0. 9) ,but there was no significant difference in the allele frequency of asthmatics compared with healthy group,(3)The frequency of Glu/Glu genotype in severe asthma was lower than stable asthma group(P<0. 05). Conclusion These results suggesteded that β2-AR 27 locus genetic polymorphisms is correlated with asthma,and the Glu27 could have the protective effect to the airway.