中华临床感染病杂志
中華臨床感染病雜誌
중화림상감염병잡지
CHINESE JOURNAL OF CLINICAL INFECTIOUS DISEASES
2009年
2期
82-85
,共4页
黄丽丽%赵龙凤%窦永青%李红%郝彦琴
黃麗麗%趙龍鳳%竇永青%李紅%郝彥琴
황려려%조룡봉%두영청%리홍%학언금
内毒素类%醛固酮%肝星状细胞%肝纤维化%核转录因子κB p65
內毒素類%醛固酮%肝星狀細胞%肝纖維化%覈轉錄因子κB p65
내독소류%철고동%간성상세포%간섬유화%핵전록인자κB p65
Endotoxins%Aldosteron%Hepatic stellate cell%Liver fibrosis%Nuclear factor-κB p65
目的 探讨内毒素对大鼠肝星状细胞(HSC)核转录因子KB(NF-κB)p65 mRNA表达及醛固酮分泌的影响.方法 将培养后的HSC-T6分为内毒素组和对照组,内毒素组采用1.0 μg/mL的内毒素进行处理.放射免疫法检测HSC分泌醛固酮的情况,一步法RT-PCR检测HSC表达NF-κB 065 mRNA的水平.结果 内毒素作用6、12、24和48 h时,醛固酮分泌显著高于对照组(t值分别为3.063、4.577、6.847和9.317,P值均<0.05),NF-κB 065 mRNA的表达与对照组比较,差异有统计学意义(t值分别为5.155、6.095、7.875和9.313,P值均<0.01).HSC中醛固酮的分泌与NF-κB p65 mRNA的表达旱正相关(r=0.889,P<0.01).结论 内毒素可以上调HSC分泌醛固酮并使NF-κB p65 mRNA的表达上调,这种效果随着内毒素作用时间的延长而更为显著,可能是内毒素致肝纤维化形成的机制之一.
目的 探討內毒素對大鼠肝星狀細胞(HSC)覈轉錄因子KB(NF-κB)p65 mRNA錶達及醛固酮分泌的影響.方法 將培養後的HSC-T6分為內毒素組和對照組,內毒素組採用1.0 μg/mL的內毒素進行處理.放射免疫法檢測HSC分泌醛固酮的情況,一步法RT-PCR檢測HSC錶達NF-κB 065 mRNA的水平.結果 內毒素作用6、12、24和48 h時,醛固酮分泌顯著高于對照組(t值分彆為3.063、4.577、6.847和9.317,P值均<0.05),NF-κB 065 mRNA的錶達與對照組比較,差異有統計學意義(t值分彆為5.155、6.095、7.875和9.313,P值均<0.01).HSC中醛固酮的分泌與NF-κB p65 mRNA的錶達旱正相關(r=0.889,P<0.01).結論 內毒素可以上調HSC分泌醛固酮併使NF-κB p65 mRNA的錶達上調,這種效果隨著內毒素作用時間的延長而更為顯著,可能是內毒素緻肝纖維化形成的機製之一.
목적 탐토내독소대대서간성상세포(HSC)핵전록인자KB(NF-κB)p65 mRNA표체급철고동분비적영향.방법 장배양후적HSC-T6분위내독소조화대조조,내독소조채용1.0 μg/mL적내독소진행처리.방사면역법검측HSC분비철고동적정황,일보법RT-PCR검측HSC표체NF-κB 065 mRNA적수평.결과 내독소작용6、12、24화48 h시,철고동분비현저고우대조조(t치분별위3.063、4.577、6.847화9.317,P치균<0.05),NF-κB 065 mRNA적표체여대조조비교,차이유통계학의의(t치분별위5.155、6.095、7.875화9.313,P치균<0.01).HSC중철고동적분비여NF-κB p65 mRNA적표체한정상관(r=0.889,P<0.01).결론 내독소가이상조HSC분비철고동병사NF-κB p65 mRNA적표체상조,저충효과수착내독소작용시간적연장이경위현저,가능시내독소치간섬유화형성적궤제지일.
Objecfive To investigate the effects of endotoxin on nuclear factor-κB p65(NF-κB p65)mRNA expression and ahtosteron secretion in rat hepatic stellate cells(HSCs).Methods Cultured rat HSCs(HSC-T6)were divided into endotoxin-treated group and control group.Cells in endotoxin-treated group were exposure to 1 mg/ml.endotoxin.Aldosteron secretions of HSCs were determined by radioimmunoassay,and NF-κB p65 mRNA expressions of HSCs were detected by one-step RT-PCR.Results At 6,12,24 and 48 h,aldosteron secretions in endotoxin-treated group were significantly hisher than those in the control group(t=3.063,4.577,6.847 and 9.317,P<0.05),and the expressions of NF-κB p65 mRNA in endotoxin-treated group were also higher than those in control group(t=5.155,6.095,7.875 and 9.313,P<0.01).Aldosteron secretions and NF-κB p65 mRNA expressions in HSCs displayed a positive correlation(r=0.886,P<0.01).Conclusion Endotoxin can up-regulate the aldosteron secretion and NF-κB p65 mRNA expression in rat HSCs,which may be one of the mechanisms of liver fibrosis induced by endotoxin.
