中华流行病学杂志
中華流行病學雜誌
중화류행병학잡지
CHINESE JOURNAL OF EPIDEMIOLOGY
2011年
6期
593-597
,共5页
刘芮汐%李奇志%幸琳琳%彭哲%朱朝敏
劉芮汐%李奇誌%倖琳琳%彭哲%硃朝敏
류예석%리기지%행림림%팽철%주조민
结核分枝杆菌%北京基因型%数目可变串联重复序列%间隔区寡核苷酸分型%儿童
結覈分枝桿菌%北京基因型%數目可變串聯重複序列%間隔區寡覈苷痠分型%兒童
결핵분지간균%북경기인형%수목가변천련중복서렬%간격구과핵감산분형%인동
Mycobacterium tuberculosis%Beijing genotype%Mycobaeterial interspersed repetitive unit-variable-number tandem repeat%Spoligotyping%Children
目的 评价间隔区寡核苷酸分型(Spoligotyping)和基于结核分枝杆菌散在分布数目可变串联重复序列分析(MIRU-VNTR)方法在重庆地区儿童结核病分子流行病学中的应用.方法 收集重庆地区210株儿童结核分枝杆菌(MTB)临床分离株,应用上述两种分型方法进行比较分析.结果 采用Spoligotyping分型方法,210株菌可分为2个基因群44种基因型,其中最大的1个基因群即北京家族(北京基因型)含有130株菌(61.90%).采用MIRU-VNTR分析发现24个位点的多态性差异较大,不同MIRU位点组合(12、15和24位点)的分辨率指数依次升高,后两个组合的差异是由位点ETR-B引起.各位点和各位点组合在北京家族菌株中的分辨率指数均高于非北京家族菌株.结论 重庆地区儿童MTB具有明显的基因多态性,其主要流行型为北京家族.在结核病原学监测中,可先采用Spoligotyping,再对成簇菌株进行15位点与ETR-B组合二次分型的联合分型策略,可提高分子流行病学调查效果.
目的 評價間隔區寡覈苷痠分型(Spoligotyping)和基于結覈分枝桿菌散在分佈數目可變串聯重複序列分析(MIRU-VNTR)方法在重慶地區兒童結覈病分子流行病學中的應用.方法 收集重慶地區210株兒童結覈分枝桿菌(MTB)臨床分離株,應用上述兩種分型方法進行比較分析.結果 採用Spoligotyping分型方法,210株菌可分為2箇基因群44種基因型,其中最大的1箇基因群即北京傢族(北京基因型)含有130株菌(61.90%).採用MIRU-VNTR分析髮現24箇位點的多態性差異較大,不同MIRU位點組閤(12、15和24位點)的分辨率指數依次升高,後兩箇組閤的差異是由位點ETR-B引起.各位點和各位點組閤在北京傢族菌株中的分辨率指數均高于非北京傢族菌株.結論 重慶地區兒童MTB具有明顯的基因多態性,其主要流行型為北京傢族.在結覈病原學鑑測中,可先採用Spoligotyping,再對成簇菌株進行15位點與ETR-B組閤二次分型的聯閤分型策略,可提高分子流行病學調查效果.
목적 평개간격구과핵감산분형(Spoligotyping)화기우결핵분지간균산재분포수목가변천련중복서렬분석(MIRU-VNTR)방법재중경지구인동결핵병분자류행병학중적응용.방법 수집중경지구210주인동결핵분지간균(MTB)림상분리주,응용상술량충분형방법진행비교분석.결과 채용Spoligotyping분형방법,210주균가분위2개기인군44충기인형,기중최대적1개기인군즉북경가족(북경기인형)함유130주균(61.90%).채용MIRU-VNTR분석발현24개위점적다태성차이교대,불동MIRU위점조합(12、15화24위점)적분변솔지수의차승고,후량개조합적차이시유위점ETR-B인기.각위점화각위점조합재북경가족균주중적분변솔지수균고우비북경가족균주.결론 중경지구인동MTB구유명현적기인다태성,기주요류행형위북경가족.재결핵병원학감측중,가선채용Spoligotyping,재대성족균주진행15위점여ETR-B조합이차분형적연합분형책략,가제고분자류행병학조사효과.
Objective To evaluate the application of spacer oligonucleotide typing (Spoligotyping) and mycobaeterial interspersed repetitive unit-variable-number tandem repeat (MIRU-VNTR) analysis in the molecular-epidemiological study of tuberculosis and to discuss the characteristics of pediatric Mycobacterium (M.) tuberculosis strains in Chongqing. Methods M. tuberculosis strains isolated and typed by Spoligotyping and MIRU-VNTR respectively, from the children patients in Chongqing and to compare the results from both methods, epidemiologically. Results By means of Spoligotyping, 210 clinical isolates were divided into 2 gene groups, displaying 44 genotypes. Among them, the biggest group was M. tuberculosis Beijing family, including 130 strains (61.90%) ,using the Spoligotyping. From the results of MIRU-VNTR, 24 loci showed different polymorphism and the HGI of different loci set (12 old loci, 15 basic loci and 24-loci set) increased accordingly. The subtle difference in HGI was originated from one locus ETR-B, which was included in the 24-locus system. The diversity of each loci and MIRU-VNTR set for non-Beijing genotype strains was higher than that of the Beijing genotype strains. Conclusion In this study, it was preliminarily confirmed the existence of high polymorphism of M. tuberculosis while the Beijing Family was the main genotype and main prevalent strain in children of Chongqing area. Spoligotyping prior to 15-locus with ETR-B combination seemed more suitable for the massive epidemiological investigation of pediatric tuberculosis patients.
