CAJ | 학술논문

目的通过分离并鉴定胆囊癌和胆囊良性组织的差异表达蛋白质,以发现可能用于早期诊断或治疗的胆囊癌肿瘤标志物.方法提取人胆囊癌和胆囊良性组织(各6例)的总蛋白质,用双向电泳分离蛋白并进行比较.选择在胆囊癌组织中明显差异表达的蛋白点,行质谱分析.采用免疫组织化学EliVision法,对50例原发性胆囊癌38例慢性胆囊炎组织中膜联蛋白A3表达进行检测,分析胆囊癌中膜联蛋白A3的表达状况及其与胆囊癌临床病理指标和预后的关系.结果获得了分辨率和重复性均很好的凝胶蛋白图谱.对筛选出的在胆囊癌组织中明显差异表达的46个蛋白点,共有17种蛋白质被成功鉴定,其中在胆囊癌组织中高表达9个,低表达8个.膜联蛋白A3在胆囊癌组织中表达的阳性率明显高于慢性胆囊炎组织,差异有统计学意义(74.0%:21.1%,P<0.01),其表达水平与胆囊癌患者年龄、性别及组织学类型等因素均无明显关系(P>0.05),但高表达与胆囊癌的低分级(40.0%:82.5%,P<0.05)、淋巴结或远处转移(40.9%:100.0%,P<0.05)以及术后生存时间减少(50.0%:93.8%,P<0.05)有明显关系.结论胆囊癌组织蛋白与胆囊良性组织存在明显的差异.膜联蛋白A3可能对胆囊癌的发生、发展有作用.
목적 통과분리병감정담낭암화담낭량성조직적차이표체단백질,이발현가능용우조기진단혹치료적담낭암종류표지물.방법 제취인담낭암화담낭량성조직(각6례)적총단백질,용쌍향전영분리단백병진행비교.선택재담낭암조직중명현차이표체적단백점,행질보분석.채용면역조직화학EliVision법,대50례원발성담낭암38례만성담낭염조직중막련단백A3표체진행검측,분석담낭암중막련단백A3적표체상황급기여담낭암림상병리지표화예후적관계.결과 획득료분변솔화중복성균흔호적응효단백도보.대사선출적재담낭암조직중명현차이표체적46개단백점,공유17충단백질피성공감정,기중재담낭암조직중고표체9개,저표체8개.막련단백A3재담낭암조직중표체적양성솔명현고우만성담낭염조직,차이유통계학의의(74.0%:21.1%,P<0.01),기표체수평여담낭암환자년령、성별급조직학류형등인소균무명현관계(P>0.05),단고표체여담낭암적저분급(40.0%:82.5%,P<0.05)、림파결혹원처전이(40.9%:100.0%,P<0.05)이급술후생존시간감소(50.0%:93.8%,P<0.05)유명현관계.결론 담낭암조직단백여담낭량성조직존재명현적차이.막련단백A3가능대담낭암적발생、발전유작용.
Objective To explore the potential molecular targets for diagnosis and treatment of gallbladder cancer by analyzing and comparing the proteomes expressed in human gallbladder cancer and benign gallbladder tissues. Methods The proteins expressed were analyzed using two-dimensional gel electrophoresis. The differentially expressed proteins in tumors were also analyzed by mass spectrometry (MS). AnnexinA3 expression was examined by streptavidin peroxidase immunohistochemical technique on paraffin-embedded tissue sections from SO patients of gallbladder cancer and 38 cases of chronic eholecystitis. Results Protein extracts of individual sample in each type of tissues were separated on two-dimensional gels. There were forty six differentially expressed proteins in the tissue samples of gallbladder cancer. Seventeen proteins were successfully identified by MS, in which nine proteins were overexpressed in tumors and the other eight proteins were underexpressed. The positive expression rates of annexinA3 in gallbladder cancer was significantly higher than that in chronic cholecystitis, and the difference was statistically significant (74. 0% vs 21. 1% , P < 0. 01). In the gallbladder cancer, no correlation was obtained between annexinA3 and age, gender or histologicl type (P > 0.05), but overexpression of annexinA3 correlated significantly with those cases with a lower histological grading (40. 0% vs 82. 5% ,P < 0. 05);lymph node or distant metastasis (40. 9% vs 100% , P < 0. 05) ;or a shorter survival time after operation (50. 0% vs 93. 8% , P < 0. 05). Conclusions Significant discrepancies in protein expression exist among gallbladder cancer and benign gallbladder tissues. AnnexinA3 plays an important role in the initiation and progression of human gallbladder cancer.