中华实验和临床病毒学杂志
中華實驗和臨床病毒學雜誌
중화실험화림상병독학잡지
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
2010年
2期
104-106
,共3页
李钟燮%孟繁平%申钢铁%金丹%冯相伟
李鐘燮%孟繁平%申鋼鐵%金丹%馮相偉
리종섭%맹번평%신강철%금단%풍상위
肝炎病毒%基因型%人种群
肝炎病毒%基因型%人種群
간염병독%기인형%인충군
Hepacivirus%Genotype%Ethnic groups
目的 分析吉林省延边地区朝鲜族与汉族丙型肝炎病毒(HCV)基因型特征.方法 采用荧光定量PCR方法和探针反向杂交技术对延边地区收治的62例朝鲜族和57例汉族慢性丙型肝炎患者进行HCV病毒载量检测和HCV基因型分析,比较HCV基因型在朝鲜族与汉族之间的分布差异,并分析HCV基因型与病毒载量、2型糖尿病和肝病严重程度之间的相关性.结果 朝鲜族HCV基因型显示,1b型占45.16%(28/62)、2a型占38.71%(24/62)、未分型占16.13%((10/62);汉族HCV基因型显示,1b型占45.61%(26/57),2a型占40.35%(23/57),未分型占14.14%(8/57),朝、汉族之间各基因型分布差异无统计学意义(P＞0.05).HCV各基因型之间HCV病毒载量差异和合并2型糖尿病分布差异之间无统计学意义(P＞0.05),而HCV 1b型在中、重度慢性丙型肝炎患者与轻度慢性丙型肝炎患者中所占的比例差异具有统计学意义(P＜0.05).结论 ①吉林省延边地区HCV基因型中以1b型最多,2a型次之,还有少数其他基因型;②HCV基因型在朝、汉民族之间分布无差异;③HCV基因型与HCV-RNA载量无关;④HCV基因型在合并糖尿病者与未合并糖尿病者之间分布无差异;⑤1b型HCV感染者病情相对较重.
目的 分析吉林省延邊地區朝鮮族與漢族丙型肝炎病毒(HCV)基因型特徵.方法 採用熒光定量PCR方法和探針反嚮雜交技術對延邊地區收治的62例朝鮮族和57例漢族慢性丙型肝炎患者進行HCV病毒載量檢測和HCV基因型分析,比較HCV基因型在朝鮮族與漢族之間的分佈差異,併分析HCV基因型與病毒載量、2型糖尿病和肝病嚴重程度之間的相關性.結果 朝鮮族HCV基因型顯示,1b型佔45.16%(28/62)、2a型佔38.71%(24/62)、未分型佔16.13%((10/62);漢族HCV基因型顯示,1b型佔45.61%(26/57),2a型佔40.35%(23/57),未分型佔14.14%(8/57),朝、漢族之間各基因型分佈差異無統計學意義(P＞0.05).HCV各基因型之間HCV病毒載量差異和閤併2型糖尿病分佈差異之間無統計學意義(P＞0.05),而HCV 1b型在中、重度慢性丙型肝炎患者與輕度慢性丙型肝炎患者中所佔的比例差異具有統計學意義(P＜0.05).結論 ①吉林省延邊地區HCV基因型中以1b型最多,2a型次之,還有少數其他基因型;②HCV基因型在朝、漢民族之間分佈無差異;③HCV基因型與HCV-RNA載量無關;④HCV基因型在閤併糖尿病者與未閤併糖尿病者之間分佈無差異;⑤1b型HCV感染者病情相對較重.
목적 분석길림성연변지구조선족여한족병형간염병독(HCV)기인형특정.방법 채용형광정량PCR방법화탐침반향잡교기술대연변지구수치적62례조선족화57례한족만성병형간염환자진행HCV병독재량검측화HCV기인형분석,비교HCV기인형재조선족여한족지간적분포차이,병분석HCV기인형여병독재량、2형당뇨병화간병엄중정도지간적상관성.결과 조선족HCV기인형현시,1b형점45.16%(28/62)、2a형점38.71%(24/62)、미분형점16.13%((10/62);한족HCV기인형현시,1b형점45.61%(26/57),2a형점40.35%(23/57),미분형점14.14%(8/57),조、한족지간각기인형분포차이무통계학의의(P＞0.05).HCV각기인형지간HCV병독재량차이화합병2형당뇨병분포차이지간무통계학의의(P＞0.05),이HCV 1b형재중、중도만성병형간염환자여경도만성병형간염환자중소점적비례차이구유통계학의의(P＜0.05).결론 ①길림성연변지구HCV기인형중이1b형최다,2a형차지,환유소수기타기인형;②HCV기인형재조、한민족지간분포무차이;③HCV기인형여HCV-RNA재량무관;④HCV기인형재합병당뇨병자여미합병당뇨병자지간분포무차이;⑤1b형HCV감염자병정상대교중.
Objective To investigate the characteristics of HCV genotypes of Han and Korean in Yanbian area of Jilin Province.Methods The HCV RNA load and genotypes of the 119 chronic hepatitis C patients in Yanbian area of Jilin Province were determined by real-time PCR and LiPA.The differences of the HCV genotypes in Han and Korean cases,in severity of the diseases,in HCV-RNA load,and in the relation with type 2 diabetes mellitus were analyzed.Results There was no significant difference in the distribution of each HCV genotype between Han and Korean patients (P＞0.05) with chronic hepatitis C.The difference between HCV genotype and HCV-RNA load was not significant (P＞0.05).With and without type 2 diabetes mellitus in these patients.The distribution of HCV genotype was also not significandy different (P＞0.05).The type 1b of HCV genotype in the moderate to severe chronic hepatitis C patients accounted for 58.06%.It was different compared with mild chronic hepatitis C patients (P＜0.05).Conclusion ①The type 1b is the most popular HCV genotype in Yanbian area of Jilin Province,type 2a is the second and there are still a few other genotypes.②There is no significant difference in the distribution of HCV genotypes between Han and Korean cases.③The HCV genotypes has nothing to do with the load of HCV-RNA.④The distribution of HCV genotypes in chronic hepatitis C patients with and without diabetes mellitus is not significantly different.⑤Type 1b of HCV infection is relatively severe.
