中国兽医学报
中國獸醫學報
중국수의학보
CHINESE JOURNAL OF VETERINARY SCIENCE
2009年
5期
603-609
,共7页
ZHU Cheng-ru%MAO Ying%Edgar C. Boedeker%冯书章
ZHU Cheng-ru%MAO Ying%Edgar C. Boedeker%馮書章
ZHU Cheng-ru%MAO Ying%Edgar C. Boedeker%풍서장
兔肠致病性大肠杆菌%ifA基因%道定居
兔腸緻病性大腸桿菌%ifA基因%道定居
토장치병성대장간균%ifA기인%도정거
rEPEC%lifA%colonization
兔肠致病性大肠杆菌(rEPEC)菌株RDEC-1的基因组中lifA基因与LEE(Locus for enterocyte effacement)致病岛相毗邻.本试验通过DNA序列分析、基因打靶技术、细胞因子检测以及动物试验,分析lifA基因完整核苷酸序列及其生物学功能.结果表明,RDEC-1的lifA基因的核苷酸序列与人肠致病性大肠杆菌的完全相同;ifA基因具有降低家兔外周血单核细胞IL-2表达的作用.与野生型菌株RDEC-1相比,被定点敲除lifA基因的RDEC-1突变株(RDEC-1△lifA)口服接种家兔后,排菌量明显降低.利用野生型RDEC-1和RDEC-1△lifA基因缺失菌株同时口服接种家兔,从粪便中分离细菌,结果显示野生型RDEC-1是优势菌,而RDEC-1△lifA基因缺失菌数量极少.RDEC-1△lifA基因缺失菌株和野生型RDEC-1都能引起特征性家兔肠道上皮的黏附与细胞脱落病变(A/Elesion).表明rEPEC的lifA基因在免疫调节和细菌的肠道定居中起重要作用,这为研究lifA基因的生物学功能提供了直接证据.
兔腸緻病性大腸桿菌(rEPEC)菌株RDEC-1的基因組中lifA基因與LEE(Locus for enterocyte effacement)緻病島相毗鄰.本試驗通過DNA序列分析、基因打靶技術、細胞因子檢測以及動物試驗,分析lifA基因完整覈苷痠序列及其生物學功能.結果錶明,RDEC-1的lifA基因的覈苷痠序列與人腸緻病性大腸桿菌的完全相同;ifA基因具有降低傢兔外週血單覈細胞IL-2錶達的作用.與野生型菌株RDEC-1相比,被定點敲除lifA基因的RDEC-1突變株(RDEC-1△lifA)口服接種傢兔後,排菌量明顯降低.利用野生型RDEC-1和RDEC-1△lifA基因缺失菌株同時口服接種傢兔,從糞便中分離細菌,結果顯示野生型RDEC-1是優勢菌,而RDEC-1△lifA基因缺失菌數量極少.RDEC-1△lifA基因缺失菌株和野生型RDEC-1都能引起特徵性傢兔腸道上皮的黏附與細胞脫落病變(A/Elesion).錶明rEPEC的lifA基因在免疫調節和細菌的腸道定居中起重要作用,這為研究lifA基因的生物學功能提供瞭直接證據.
토장치병성대장간균(rEPEC)균주RDEC-1적기인조중lifA기인여LEE(Locus for enterocyte effacement)치병도상비린.본시험통과DNA서렬분석、기인타파기술、세포인자검측이급동물시험,분석lifA기인완정핵감산서렬급기생물학공능.결과표명,RDEC-1적lifA기인적핵감산서렬여인장치병성대장간균적완전상동;ifA기인구유강저가토외주혈단핵세포IL-2표체적작용.여야생형균주RDEC-1상비,피정점고제lifA기인적RDEC-1돌변주(RDEC-1△lifA)구복접충가토후,배균량명현강저.이용야생형RDEC-1화RDEC-1△lifA기인결실균주동시구복접충가토,종분편중분리세균,결과현시야생형RDEC-1시우세균,이RDEC-1△lifA기인결실균수량겁소.RDEC-1△lifA기인결실균주화야생형RDEC-1도능인기특정성가토장도상피적점부여세포탈락병변(A/Elesion).표명rEPEC적lifA기인재면역조절화세균적장도정거중기중요작용,저위연구lifA기인적생물학공능제공료직접증거.
The rabbit enteropathogenic E. coli (rEPEC) strain RDEC-1 possesses a lifA homologue adjacent to the LEE pathogenicity island. To study the entire nucleotide sequence and biological function of lifA,the DNA sequence and biological function of RDEC-1 lifA were analysed with gene cloning,gene knock-out and in vivo virulence examination. The result showed that the entire coding sequence of the lifA of RDEC-1 shares nearly absolute homology with the lifA of human isolates. RDEC-1 lifA inhibited IL-2 expression in stimulated rabbit peripheral blood mononuclear cells. We further demonstrated significant reduction in fecal bacterial shedding by RDEC-1 derivative lifA mutant when compared with its parent strain. In a competitive study when rabbits were inoculated with a combination of the WT and the mutant, the WT was the predominant bacteria recovered from fecal samples, while fewer mutant bacteria were recovered. However,the lifA mutant is able to induce A/E type of lesions as efficient as the parent strain. The data provide direct evidence that lifA of rEPEC plays a role in immunomodulation and in in vivo colonization in the intestinal tract.