中国组织工程研究与临床康复
中國組織工程研究與臨床康複
중국조직공정연구여림상강복
JOURNAL OF CLINICAL REHABILITATIVE TISSUE ENGINEERING RESEARCH
2009年
46期
9041-9044
,共4页
田力%范妮娜%田晓晔%梁晓鹏%汪卓%李娜
田力%範妮娜%田曉曄%樑曉鵬%汪卓%李娜
전력%범니나%전효엽%량효붕%왕탁%리나
骨髓间充质干细胞%软骨%关节
骨髓間充質榦細胞%軟骨%關節
골수간충질간세포%연골%관절
背景:骨髓间充质干细胞在不同诱导条件下具有向中胚层组织细胞如成骨细胞、成软骨细胞、肌细胞、脂肪细胞等分化的能力.目的:验证用组织工程方法诱导分化骨髓间充质干细胞修复兔关节软骨损伤的效果.设计、时间及地点:随机对照动物实验,于2005-05-03/2007-12-30在沈阳医学院临床中心实验室完成.材料:20只两三月龄的健康新西兰白兔,雌雄不限.方法:①诱导分化体外培养的兔骨髓间充质干细胞.实验组加入地塞米松、碱性成纤维细胞生长因子、维生素C培养1周,再将转化生长因子β替换碱性成纤维细胞生长因子培养3周;以不加诱导剂细胞做对照.②取20只兔.建立膝关节软骨缺损模型,随机分为3组.实验组10只膝关节内植入经诱导的骨髓间充质干细胞;对照组植入未经诱导的骨髓间充质干细胞:空白对照组植入生理盐水.分别于术后2,4,6,8周时处死实验组处死2只,对照组和空白对照组各处死1只进行各项指标检测.主要观察指标:①细胞形态学.②碱性磷酸酶活性的测定.③大体标本观察.④X射线观察.⑤组织切片观察.结果:①经诱导的骨髓间充质干细胞,细胞形态发生明显变化,逐渐由长梭形变为多角形,类似于软骨细胞样形态.②骨髓间充质干细胞经诱导4周后,其碱性磷酸酶活性明显增强(P<0.05).③术后8周,实验组标本修复组织表面光滑,与周围软骨间界限模糊不清:X射线表现为关节间隙变宽,软骨下骨质囊变得到改善:组织切片观察显示与正常软骨细胞基本上一致.结论:自体间充质干细胞移植可修复关节软骨损伤.
揹景:骨髓間充質榦細胞在不同誘導條件下具有嚮中胚層組織細胞如成骨細胞、成軟骨細胞、肌細胞、脂肪細胞等分化的能力.目的:驗證用組織工程方法誘導分化骨髓間充質榦細胞脩複兔關節軟骨損傷的效果.設計、時間及地點:隨機對照動物實驗,于2005-05-03/2007-12-30在瀋暘醫學院臨床中心實驗室完成.材料:20隻兩三月齡的健康新西蘭白兔,雌雄不限.方法:①誘導分化體外培養的兔骨髓間充質榦細胞.實驗組加入地塞米鬆、堿性成纖維細胞生長因子、維生素C培養1週,再將轉化生長因子β替換堿性成纖維細胞生長因子培養3週;以不加誘導劑細胞做對照.②取20隻兔.建立膝關節軟骨缺損模型,隨機分為3組.實驗組10隻膝關節內植入經誘導的骨髓間充質榦細胞;對照組植入未經誘導的骨髓間充質榦細胞:空白對照組植入生理鹽水.分彆于術後2,4,6,8週時處死實驗組處死2隻,對照組和空白對照組各處死1隻進行各項指標檢測.主要觀察指標:①細胞形態學.②堿性燐痠酶活性的測定.③大體標本觀察.④X射線觀察.⑤組織切片觀察.結果:①經誘導的骨髓間充質榦細胞,細胞形態髮生明顯變化,逐漸由長梭形變為多角形,類似于軟骨細胞樣形態.②骨髓間充質榦細胞經誘導4週後,其堿性燐痠酶活性明顯增彊(P<0.05).③術後8週,實驗組標本脩複組織錶麵光滑,與週圍軟骨間界限模糊不清:X射線錶現為關節間隙變寬,軟骨下骨質囊變得到改善:組織切片觀察顯示與正常軟骨細胞基本上一緻.結論:自體間充質榦細胞移植可脩複關節軟骨損傷.
배경:골수간충질간세포재불동유도조건하구유향중배층조직세포여성골세포、성연골세포、기세포、지방세포등분화적능력.목적:험증용조직공정방법유도분화골수간충질간세포수복토관절연골손상적효과.설계、시간급지점:수궤대조동물실험,우2005-05-03/2007-12-30재침양의학원림상중심실험실완성.재료:20지량삼월령적건강신서란백토,자웅불한.방법:①유도분화체외배양적토골수간충질간세포.실험조가입지새미송、감성성섬유세포생장인자、유생소C배양1주,재장전화생장인자β체환감성성섬유세포생장인자배양3주;이불가유도제세포주대조.②취20지토.건립슬관절연골결손모형,수궤분위3조.실험조10지슬관절내식입경유도적골수간충질간세포;대조조식입미경유도적골수간충질간세포:공백대조조식입생리염수.분별우술후2,4,6,8주시처사실험조처사2지,대조조화공백대조조각처사1지진행각항지표검측.주요관찰지표:①세포형태학.②감성린산매활성적측정.③대체표본관찰.④X사선관찰.⑤조직절편관찰.결과:①경유도적골수간충질간세포,세포형태발생명현변화,축점유장사형변위다각형,유사우연골세포양형태.②골수간충질간세포경유도4주후,기감성린산매활성명현증강(P<0.05).③술후8주,실험조표본수복조직표면광활,여주위연골간계한모호불청:X사선표현위관절간극변관,연골하골질낭변득도개선:조직절편관찰현시여정상연골세포기본상일치.결론:자체간충질간세포이식가수복관절연골손상.
BACKGROUND:Under different induction conditions,bone marrow mesenchymal stem cells can differentiate into the mesodermal tissues such as osteoblasts,chondroblasts,muscle cells,adipocytes and so on.OBJECTIVE:To verify the effect on repairing the rabbit articular cartilage injury using bone marrow mesenchymal stern cells (MSCs)induced by tissue engineering method.DESIGN,TIME AND SETTING:Randomized controlled animal experiment was performed in the Clinical Center Laboratory of Shenyang Medical College between May 2005 and December 2007.MATERIALS:Twenty health New Zealand rabbits,irrespective of genders,aged 2-3 months,were used.METHODS:①Rabbit bone marrow MSCs were cultured in vitro,experiment group was cultured for one week withdexamethasone,basic fibroblast growth factor and vitamin C,then for additional 3 weeks with transforming growth factor-β instead of basic fibroblast growth factor;calls without inductors served as controls;②Twenty rabbits were used to establish knee articular cartilage defect models,which were then divided into three groups at random. Experiment group (n=5) was transplanted with the induced bone marrow MSCs;control group with the non-induced cells;blank control group with saline. At 2,4,6,8 weeks postoperation,two rabbits in the experiment group were killed,while one animal in control group and blank control group was killed for the index determination.MAIN OUTCOME MEASURES:①Cell morphology. ②Alkaline phosphatase activities.③General observation. ④X-ray observation.⑤Histological observation.RESULTS:①The morphology of the induced bone marrow MSCs was changed,from long fusiform to polygon,which was similar to cartilage calls-like morphology.②After the bone marrow MSCs were induced for 4 weeks,the alkaline phosphatase activities were obviously enhanced(P<0.05).③Eight weeks after transplantation,the specimens in the experiment group exhibited smooth surface and unclear outlines with surrounding cartilage;X-ray results showed joint space broadened,subchondral bone sack was improved;histological slices observation revealed similarity with normal chondrocytes.CONCLUSION:Autologous MSCs transplantation can repair articular cartilage injury.