CAJ | 학술논문

目的用透射电镜观察水杨酸钠引起耳鸣大鼠听皮层突触形态的变化.方法成年健康雄性白色Wistar大鼠32只,随机平分为2组.第一组腹腔注射水杨酸钠350 mg/kg,第二组腹腔注射等量生理盐水,均在每天上午9点注射,持续30天.两组所处饲养环境(包括环境噪声、12小时昼夜节律、饮食等)完全相同.1月后快速处死.取双侧听皮层组织进行透射电镜观察.结果与对照组相比,水杨酸组突触数量明显增加,许多突触形态由平型变成凹型或U型.突触界面曲率增加,突触面积增大,活性区增加,每一活性区的长度增加,突触间隙明显增宽,突触后膜致密物质厚度增加.U型突触直径平均为(1.7±O.23)μm,平直型突触直径平均为(O.53±O.14)μm,二者比较有显著性差异(P<0.05).结论水杨酸钠能够引起听皮层突触形态的改变,这种改变与长时程增强现象(Long-term potentiation,LTP)非常相似,有可能是耳鸣与突触可塑性以及与学习记忆相关的有力证据,值得深入研究.
목적 용투사전경관찰수양산납인기이명대서은피층돌촉형태적변화.방법 성년건강웅성백색Wistar대서32지,수궤평분위2조.제일조복강주사수양산납350 mg/kg,제이조복강주사등량생리염수,균재매천상오9점주사,지속30천.량조소처사양배경(포괄배경조성、12소시주야절률、음식등)완전상동.1월후쾌속처사.취쌍측은피층조직진행투사전경관찰.결과 여대조조상비,수양산조돌촉수량명현증가,허다돌촉형태유평형변성요형혹U형.돌촉계면곡솔증가,돌촉면적증대,활성구증가,매일활성구적장도증가,돌촉간극명현증관,돌촉후막치밀물질후도증가.U형돌촉직경평균위(1.7±O.23)μm,평직형돌촉직경평균위(O.53±O.14)μm,이자비교유현저성차이(P<0.05).결론 수양산납능구인기은피층돌촉형태적개변,저충개변여장시정증강현상(Long-term potentiation,LTP)비상상사,유가능시이명여돌촉가소성이급여학습기억상관적유력증거,치득심입연구.
Objective To study prolonged effects of salicylic acid on the ultrastructure of synapses in the auditory cortex in a rat model of tinnitus. Methods Sixteen adult male white rats were randomly selected to be treated with peri toneal salicylic acid (300 mg/kg body weight) or with equivalent volume of normal saline for 30 days. Animals in both groups were maintained under the same conditions. Synapses in the auditory cortex were studied under a transmission electron microscope. Results Compared to the control group, salicylic acid treated animals showed increased number of synapses with wideer clefts, thicker postsynaptic densities, longer active zones, and increased synaptic interface curvature (p < 0.05). Conclusion The results suggest that Salicylic acid induce changes of synapses in rats. Such changes corre late to long-term potentiation (LTP) following intensive stimulation The results provide a strong evidence for the relation between tinnitus and learning and memory.