CAJ | 학술논문

目的:探讨桃核承气汤对四氯化碳诱导的大鼠肝损伤的保护作用.方法:30只大鼠分为正常对照组,模型组,大、小剂量桃核承气汤组和水飞蓟素组.桃核承气汤组灌胃桃核承气汤(0.3 g/kg或0.5 g/kg),水飞蓟素组灌胃水飞蓟素25 mg/kg.灌胃后1 h腹腔注射四氯化碳1.5 mL/kg.注射24 h后将大鼠处死,检测肝组织病理学改变,血液中天门冬氨酸氨基转移酶(aspartate amino transferase,AST)、丙氨酸氨基转移酶(alanine aminotransferase,ALT)活性及脂质过氧化程度.结果:桃核承气汤不仅明显降低血液中AST及ALT活性,抑制四氯化碳引起的肝脏脂质过氧化,并且增加肝脏中谷胱甘肽含量;组织病理分析发现桃核承气汤可抑制脂质堆积,肝细胞坏死及淋巴细胞浸润.结论:桃核承气汤具有类抗氧化物之作用,对肝损伤有保护作用,甚至功能优于水飞蓟素.
목적:탐토도핵승기탕대사록화탄유도적대서간손상적보호작용.방법:30지대서분위정상대조조,모형조,대、소제량도핵승기탕조화수비계소조.도핵승기탕조관위도핵승기탕(0.3 g/kg혹0.5 g/kg),수비계소조관위수비계소25 mg/kg.관위후1 h복강주사사록화탄1.5 mL/kg.주사24 h후장대서처사,검측간조직병이학개변,혈액중천문동안산안기전이매(aspartate amino transferase,AST)、병안산안기전이매(alanine aminotransferase,ALT)활성급지질과양화정도.결과:도핵승기탕불부명현강저혈액중AST급ALT활성,억제사록화탄인기적간장지질과양화,병차증가간장중곡광감태함량;조직병리분석발현도핵승기탕가억제지질퇴적,간세포배사급림파세포침윤.결론:도핵승기탕구유류항양화물지작용,대간손상유보호작용,심지공능우우수비계소.
Objective: To clarify the efficacy of Taohe Chengqi Tang (THCQT), a compound traditional Chinese herbal medicine, in protecting liver damage induced by carbon tetrachloride (CCI4) in rats.Methods: Thirty male Wistar rats were divided into normal control group, untreated group, low-dose THCQT group (receiving 0. 3 g/kg of THCQT), high-dose THCQT group (receiving 0. 5 g/kg of THCQT), and positive control group (receiving silymarin 25 mg/kg). All testing substances were orally administered 1 hour before the intraperitoneal injection of CCI4 (1.5 mL/kg). Twenty-four hours after CCI4 injection, the rats were sacrificed to observe liver histopathological changes, and to evaluate activities of serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT), and lipid peroxidation (LPO) and glutathione (GSH) levels in liver tissues.Results: CCI4 injection elevated the serum AST and ALT activities, but THCQT significantly reversed this effect. The increase of hepatic LPO by CCI4 was markedly reduced by THCQT. Also, this herbal mixture increased hepatic GSH in the rats. In histopathology analysis, THCQT decreased the fatty accumulation,necrosis and lymphocyte infiltration. The in vitro study in rat brain showed that LPO induced by Fe2+/ascorbic acid was dose-dependently reduced by THCQT. According to the biochemical and morphological data,THCQT could protect the liver from CCI4-induced injuries.Conclusion: THCQT seems helpful for protection of liver damage induced by chemicals depending on its anti-oxidant-like function, and THCQT is more effective than silymarin.